CONTENTS 



4. Chelating agents 52 



5. Mustard gas 52 



6. Alcohols 53 



7. Other chemicals 53 



8. Sonic vibration 54 



9. Surface denaturation 55 



10. Heat inactivation 56 



11. Hydrostatic pressure 60 



12. Osmotic shock 60 



13. Summary 61 



VI Effects of Radiations on Phage Particles 63 



1. Visible light 63 



2. Ultraviolet light 65 



a. Lethal effect 66 



b. Growth-delaying effect 68 



c. Host killing 69 



d. Interference 70 



e. Photoreactivation 70 



f Multiplicity reactivation 73 



3. Ionizing radiations 74 



4. Decay of radiophosphorus 80 



5. Summary 82 



VII Chemical Composition 83 



1. Concenti-ation and purification 83 



2. Criteria of purity 85 



3. Elementary composition 87 



4. Gross chemical components 88 



5. Amino acids, nucleotides, and bases 89 



6. Extinction coefficients of phage preparations. ... 92 



7. Phosphorus and nitrogen contents per infective 

 particle 94 



8. Summary 95 



VIII Antigenic Properties 97 



1 . Preparation of antiphage sera 98 



2. Multiplicity of phage antigens 100 



3. Neutralization of phage infectivity 101 



4. Properties of incompletely neutralized phage 

 preparations 102 



5. Complement fixation 104 



