172 



BACTERIOPHAGES 



4. Premature Lysis 



The one-step growth experiment reveals nothing about the im- 

 portant intracellular events during the latent period. In order 

 to penetrate behind the scenes, various methods for rupturing the 

 host cell before the end of the normal latent period have been 



.0= 



lO'- 



Ar*^ 



A LYSIS BY KCN -t- T6 



• CONTROL LYSIS 



■ SONIC DISRUPTION OF CELLS 



O 10 20 30 40 



MINUTES OF INCUBATION IN GROWTH MEDIUM AT 30* 



Figure 4. Maturation of phage T3 in infected bacteria. The curve marked 

 "control lysis" is the ordinary one-step growth of this phage. The other two 

 curves show the yields of infective particles released by two different methods 

 of artificial lysis. Reproduced from T. F. Anderson and A. H. Doermann, 

 1952a, ./. Gen. Physiol., 35, 657, with permission. 



