CHEMICAL INTERFERENCE WITH PHAGE GROWTH 287 



Three instances of chemical interference deserve special 

 mention. By the use of chloramphenicol to prevent protein 

 synthesis it is possible on the one hand to show a dependence of 

 DNA synthesis on prior protein synthesis, and on the other hand 

 to demonstrate the sequential synthesis of phage-precursor DNA 

 and protein. The acridines possess the remarkable property of 

 blocking maturation without preventing synthesis of phage 

 DNA and organized protein structures. Finally, by feeding 

 appropriate analogues, intact but noninfective phage particles 

 may be produced whose properties remain to be investigated. 



