Pfizer Handbook of Microbial Metabolites 



304 



Aspartic Acid 

 Glutamic Acid 

 Serine 

 Threonine 

 a-Alanine 



Valine 

 Methionine 

 Leucine 

 Isoleucine 



The free and combined amino acids of the uredospores of 

 ten wheat rust strains have been determined quantitatively.^" 



The intracellular amino acids of microorganisms have been 

 studied. Gale demonstrated the presence of such a pool in 

 Streptococcus faecalis}^ Gale and Taylor extended the investi- 

 gation to a variety of bacteria and yeasts with particular atten- 

 tion to lysine and glutamic acid.^- Fuerst studied several 

 fungi." The free intracellular amino acids of certain strains 

 of Neurospora crassa have been explored." The relative quan- 

 tities of amino acids present varied widely among the various 

 mutants. In all some 35 ninhydrin-positive substances were en- 

 countered among the 28 different strains studied. The free 

 amino acids of Staphylococcus aureus have been determined, 

 and the ability of bacteria to concentrate amino acids strikingly 

 demonstrated by comparison of the concentrations of internal 

 and external acids. ^^ 



TABLE VI 

 Free Amino Acids in Exponenfially Growing Staphylococcus aureus Cells Growing in Synfhefic 



Medium 



1" M. E. McKillican, Can. J. Chem. 38 244 (1960). 



"E. F. Gale, /. Gen. Microbiol, 1 53 (1947). 



12 E. F. Gale and E. S. Taylor, ibid. I 77 (1947); E. S. Taylor, ibid. 

 1 86 (1947). 



" R. Fuerst and J. Awapara, Texas Repts. Biol, and Med. 10 424 

 (1952). 



^^ Robert Fuerst and Robert P. Wagner, Arch. Biochem. and Bio- 

 phys. 70 311 (1957). 



15 R. Hancock, Biochim. et Biophys. Acta 28 402 (1958). 



