Pfizer Handbook of Microbial Metabolites 



342 



distinguished by formation of their dinitrophenyl derivatives. 

 The results are shown in the following table: 



TABLE III 

 Some Infraceltular Peptides of Escherichia co/i 



It has been reported that gram-negative bacteria contain 

 much less intracellular free ninhydrin-positive substances than 

 do gram-positive ones.-^ 



A basic polypeptide has been extracted from dried cells of the 

 human strain of Mycobactenum tuberculosis H.jyR,, purified, 

 crystallized and quantitatively analyzed for amino acid con- 

 stituents.-' The pure peptide showed activity in the tuberculin 

 test at least equal to that of standard old tuberculin. The amino 

 acid content was as follows, subscripts indicating number of 

 moles : 



Argio HiSi Lyso Phe, Tyr, Leu..^ Ileu^. Val- 

 Alag Glye Glus Pro- Ser^ Thr.{ Asp,, Tryi 



The molecular weight was calculated to be 7180. 



Certain polypeptides accumulate in E. coli cells grown in the 

 presence of chloramphenicol (a protein synthesis inhibitor). 

 Two of these have been isolated and purified.-^ 



-' Yuichi Yamamura, Seisi Morizawa, Atsushi Tanaka, and Kenji 

 Shojima, Proc. Jap. Acad. Sci. 35 295 (1959); Seisi Morizawa, Atsushi 

 Tanaka, Kenji Shojima and Yuichi Yamamura, Biochivi. et Biophys. 

 Acta 38 252 (1960). 



-' F. Sorm and Jifina Cerna, Collection Czechoslov. Chem. Com- 

 muTi. 25 565 (1960). 



