665 Addendum 



a pentapeptide containing 1 mole of D-phenylalanine, 2 

 moles of flf/o-threonine and 2 moles of n-alanine. The 

 lipide moiety was not entirely pure, but may be a hydroxy 

 acid of about C._.4H4hO:{. Two 0-acetyl groups are present 

 in mycoside C. 



The lipoids of mycobacteria, their chemical structures 

 and biological effects have been reviewed.^" 



132a IVIycocerosic Acid, C;{2H,.40o, isolated by Anderson and collabo- 

 rators,^'^ has been shown to be 2,4,6,8-tetramethylocta- 

 cosanoic acid:^" 



CH3— (CH:),9— CH— CHj— CH— CHj— CH— CH>— CH— COOH 

 CH3 CH3 CH3 CH3 



Indications were obtained for the presence in mycobac- 

 teria of normal chain acids with 22, 24, 26 and 28 carbon 

 atoms; 2-, 4-, 6-trimethyl-substituted acids with 25, 27 

 and 29 carbon atoms; and 2-, 4-, 6-, 8-tetramethyl-sub- 

 stituted acids with 30, 32 and 34 carbon atoms. 



Succinic, fumaric and acetic acids as well as d,l-5- 

 carboxymethylhydantoin, shown below, have been iso- 



COOCH3 

 / 

 HN — 



O N O 



H 



lated as extracellular acids from Mycobacterium ranae 

 and from M. tuberculosis H37Rv.^^ 



15 E. Lederer, Angew. Chem. 72 372 (1960). 



i«L. G. Ginger and R. J. Anderson, /. Biol. Chem. 157 203 (1945) 

 and preceding papers. 



^' Cecile Asselineau, Jean Asselineau, Ragnar Ryhage, Stina 

 Stallberg-Stenhagen and Einar Stenhagen, Acta Chem. Scand. 13 

 822 (1959). 



^^ Andrea V. Fowler, Merrill N. Camien and Max S. Dunn, J. Biol. 

 Chem. 235 1386 (1960). 



