667 Addendum 



The oxidative degradation of glycolic acid in £. coli 

 takes the following course:-" 



-2H -f Acetyl CoA 



HOCH COOH • OHC -COOH • HOOC -CH— CH,— COOH 



— Acetyl COA | 



OH 



O O +H-0 



-2H I -CO, l| +CoA 



' HOOC -C CH —COOH > CH.CCOOH Acetyl CoA-^ 



-2H, -CO: 

 Summation: OHC COOH r O: • 2CO2 h H,0 



A study has been made of the synthesis of cell mate- 

 rials from acetate by Aspergillus niger^-^' and by Esche- 

 richia coli.-''' Interrelationships of the tricarboxylic acid 

 and glyoxylic acid cycles were discussed. 



Lactobacilli produce ^-hydroxy acids other than lactic. 

 Two of these have been identified as a-hydroxy-D-isovaleric 

 and D-isocaproic acids;-'' 



CH3 CH3 



\, \ 



CH— CH— COOH CH— CH3— CH— COOH 



/I /I 



CH3 OH CH3 OH 



D-lsovaleric Acid D-isocaproic Acid 



These are growth promoters for certain strains of lacto- 

 bacilli. 



Penicilliiim atrovenetum, a /^-nitropropionic acid pro- 

 ducer,'^'' was grown on C'^-labeled fj-alanine, on NaHC'^O., 

 and on 4-C''*-D,L-aspartic acid.'' Since 96 percent of the 

 label was in the 1 -position, apparently aspartic acid was 

 incorporated as a unit. 



2^H. L. Kornberg and J. R. Sadler, Nature 185 153 ri960). 



2«' J. F. Collin and H. L. Kornberg, Biochem. J. 77 430 (1960). 



^s'-H. L. Kornberg, P. J. R. Phizackerly and J. R. Sadler, ibid. 77 

 438 (1960). 



-■' Merrill N. Camien, Andree V. Fowler and Max S. Dunn, Arch. 

 Biochem. and Biophys. S?> 408 ri959). 



■'^'H. Raistrick and A. Stossl, Biochem. J. 68 647 (1958). 



•'' A. J. Birch, B. J. McLoughlin, Herchel Smith and J. Winter, 

 Chem. and Ind., 840 (1960). 



