Pfizer Handbook of Microbial Metabolites 678 



Methymycin has been found to be biosynthesized prin- 

 cipally from propionate, although one mole of acetate 

 may be incorporated. '- 



8. Alicyclic Compounds Other Than Terpenoids and 

 Steroids 



An investigation of the biosynthesis of palitantin shows 

 that it is acetate-derived, and that neither shikimic acid 

 nor mevalonic acid are involved.'^ 



Several compounds have been isolated which may be 

 related to cycloheximide : 



302a Niromycin B, C14H21O4N (suggested), white, hygroscopic crys- 

 tals, m.p. 47-67°. 



A neutral substance produced by Streptomyces albusJ* 



302b Niromycin A, white hygroscopic, amorphous powder, m.p. 98- 

 105°. 



Positive 2,4-dinitrophenylhydrazine and Tollens tests. 

 Negative ninhydrin, FeCla, Fehlings, Benedicts, Molisch, 

 biuret, KMn04 tests. 



The effect of cycloheximide on the metabolism and 

 growth of Saccharomyces pastoriamis has been studied."'^ 



Some substances related to sarkomycin and produced 

 by the same organism were overlooked.''' These were: 



301a Sarkomycin Eo, C10H14O4, m.p. 179°. 



301c Sarkomycin Ep Ci4HisOt, m.p. 169°. 



sold Sarkomycin So, Ci4Hi,OoS, m.p. 183°, [a]n +136°. 



COOH COOH 



CH,— S— CH. 



o o 



''-A. J. Birch, E. Pride, R. W. Rickards, P. J. Thomson, J. D. 

 Dutcher, D. Perlman and C. Djerassi, Chem. and hid., 1245 (1960). 



■■^ P. Chaplen and R. Thomas, Biochem. J. 77 91 (1960). 



'* Teisuke Osato, Yutaka Morikubo and Hamao Umezawa, J. Anti- 

 biotics (Japan) 13A 110 (1960). 



"5 Bradner Wood Coursen, Dissertation Abstr. 21 (1960). 



'« Sueo Tatsuoka et al., J. Antibiotics (Japan) 9B 104 (1956). 



