689 Addendum 



line, tyrosine, valine, leucine, cysteine, aspartic acid, 

 glutamic acid, histidine, imida/.ole-4-acctic acid, argi- 

 ninc. ornithine and the incompletely characterized basic 

 red pigment of the organism, C,,Ho„0,,N^,. This was yel- 

 low in alkali, red in acid solutions and gave positive 

 Bayer and Pauly diazo tests. 



A study of the biogenesis of spermidine (entry 642) in 

 microorganisms has shown that the C4 moiety is derived 

 from putrescine (or ornithine) while the C;; chain has its 

 origin in methionine.'-'* 



Biochemical pathways in legume root nodule nitrogen 

 fixation have been reviewed.'-* 



16. Amino Acids and Related Compounds 



The lysine, methionine and tryptophan contents of a 

 number of yeasts have been surveyed.'" 



In a study of the interrelationships between folic acid 

 and cobalamin in the synthesis of methionine by extracts 

 of E. coll, it was concluded that serine is not on the route 

 of biosynthesis of the methyl group of methionine.'-" 



Discussing the two modes of lysine synthesis by lower 

 fungi, Vogel has pointed out that organisms of older evo- 

 lutionary origin follow the bacterial route.'-' These in- 

 clude eubacteria, pseudomonads and actinomycetes. As- 

 comycetous and basidiomycetous fungi use the fungal 

 pathway via a-aminoadipic acid. 



17. Polypeptides and Related Compounds 



The ostreogrycin (E-129) complex was isolated in 

 1958'-^ and reported similar to streptogramin, staphylo- 

 mycin (A-899), PA- 11 4 and mikamycin. 



E-129 A probably is identical with staphylomycin M and 



123 H. Tabor, S. M. Rosenthal and C. W. Tabor, /. Biol. Chem. 233 

 907 (1958). 



124 F. J. Bergersen, Bacteriol. Revs. 24 246 (1960). 



'-'■ G. E. N. Nelson, R. F. Anderson, R. A. Rhodes, Margaret C. 

 Shekleton and H. H. Hall, Appl. Microbiol. 8 179 (1960). 



126 R. L. Kislluk and D. D. Woods, Biochem. J. 75 467 (1960). 



12" H. J. Vogel, Biochim. et Biophijs. Acta 41 172 (1960). 



12^ S. Ball, B. Boothroyd, K. A. Lees, A. H. Raper and E. Lester 

 Smith, Biochem, J. 68 24p (1958). 



