ION-EXCHANGE CHROMATOGRAPHY 



229 



1.0 2.0 3.0 4.0 



LITERS THROUGH COLUMN 



Fig. 12. Separation of three adenylic acids and the corresponding inosinic acids by 

 anion exchange at pH 5.5 in a chloride system.'" 



Exchanger: Dowex-1-chloride, 250-500 mesh, 9.5 cm. X 0.9 cm.^. 

 Solution: 0.02 M NaCl in 0.01 M acetate buffer, pH 5.5, at 1.2ml./min. 

 Sorbed material: commercial adenylic acids (ca. 10 mg. each), partially deami- 

 nated with nitrous acid. 



LITERS THROUGH COLUMN 



Fig. 13. Separation of three adenylic acids and the corresponding inosinic acids 

 by anion exchange with formic acid.'' 



Exchanger: Dowex-1-formate, 250-500 mesh, 13 cm. X 0.74 cm.^ 

 Solution: 0.125 M formic acid, 1.5 ml./min. 

 Sorbed material: same as in Fig. 12. 



