ION-EXCHANGE CHROMATOGRAPHY 



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EiG. 19. Separation of sugars by anion exchange in a borate system. ^^ 

 Exchanger: Dowex-1 -borate, 11 cm. X 0.84 cm. 2. 

 Solution: tetraborate (K2B4O7) as shown at 1 ml./min. 

 Sorbed material: 2.5 mg. ribose, 5 mg. of other sugars in 10 ml. 0.01 M K2B4O7 



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Fig. 20. Separation of ribonucleosides by anion exchange in a chloride system 

 with borate present'" (see Figs. 3, 4, 5, 21, and 22). 



Exchanger: Dowex-1-chloride, 200-400 mesh, 11 cm. X 0.85 cm.'*. 



Solution: 0.03 M KCl + 0.02 M K2B4O7 . 



Sorbed material: ca. 2 mg. of each nucleoside in 10 ml. 0.01 M K2B4O7 . 



