238 



WALDO E. COHN 



6 08 



LITERS THROUGH COLUMN 



Fig. 21. Separation of uridine and thymidine by anion exchange in a borate sys- 

 tem^o (see Figs. 3, 4, 5, and 20). 



Exchanger: same as in Fig. 20 but borate form. 



Solutions: K2B4O7 as shown, 1 ml./min. 



Sorbed material : ca. 3 mg. each of thymidine and uridine in 10 ml. 0.01 M K2B4O7 



OOOI*NM/)M 



"oom/iLeoT 0002s wNn,oM-|o,oo2s«Nfi,(x|"NM,ci "] 



^ecgJco'se OOOliK^O, O000O,Vk^,0, 



GtUCOSE-6-PO, 

 INCRGANIC- 



"-i 



4 M OsJoT 



V 



'4 OOiWMCi 1?° 



1 Toe 



i.. 



04 08 di 02 Q< 02 06 04 08 12 5 02 04 q Ci 06 

 ELUTINC solution ( LITERS! 



Fig. 22. Separation of sugar acids and sugar phosphates by anion exchange in 

 chloride system with borate present*" (see Fig. 20). 



Exchanger: Dowex-1-chloride, ca. 300 mesh, 12 cm. X 0.86 cm. 2. 

 Solutions: chloride and tetraborate as shown at 3.5 ml./min. 

 Sorbed material: 2-10 mg. of substance as shown. 



