JL — . 04 V NH^Cl — ] 



ELUTING SOLUTIONS ILlTERSI 



F^k;. 23. Separation of isomeric ribose phosphates by anion exchange in a chloride 

 system with borate present*' (see Figs. 20, 22, and 24). 

 Exchanger: same as in Fig. 22. 



Solutions: chlorides and tetraborate as shown at 2 ml./min. 

 Sorbed material: 3-8 mg. of each substance shown in 10 ml. of 0.001 M NH4OH. 



Rib-2-P04 

 27 



Rib- 4 -PO4 



^ 



0.005 M No,S04 



Fig. 24. Separation of isomeric ribose phosphates by anion exchange in a sulfate 

 system with borate present*^ (ggg Figs. 20, 22, and 23). 

 Exchanger; same as Figs. 22 and 23, in sulfate form. 



Solutions: 0.0018 M NaaSOi + 0.0018 M Na2B407 (for ribose-4-phosphate and 

 ribose-2-phosphate) , 0.005 M Na2S04 (for ribose -3-phosphate) . 

 Sorbed material: (a) 10 mg. commercial adenylic acid, 1 g. Dowex-50-H, 1 ml. 

 H2O; heated at 100°C. for 20 min., with stirring; filtered; NH4OH added to super- 

 natant; sorbed; (b) same as (a), but doubled quantities and heated for 2 hr. 



239 



