TABLE I 



Rp Values op Purine and Pyrimidine Bases and Nucleosides 



" 86% (vol. /vol.) aq. n-butanol; Whatman No. 1 paper, descending; Markham and Smith, Biochem. J' 

 45, 294 (1949). Values for deoxyribosides are from Buchanan, 33 using n-butanol satd. with water; values fo 

 methylcytosine and hydroxymethylcytosine detd. by the reviewer. 



'' 86% (vol. /vol.) aq. n-butanol, with 5% by vol. of coned. NH3 soln. (sp. gr. 0.880) added to solvent in 

 bottom of tank; Whatman No. 1, descending; Markham and Smith, op. cit. Values for methylcytosine, hy- 

 droxymethylcytosine, inosine, and thymine deoxyriboside detd. by the reviewer. 



" n-Butanol satd. with water at about 23° 100 ml., 15 N NH4OH 1 ml.; Whatman No. 4, ascending; Rlac- 

 Nutt, Biochem. J. 50, 384 (1952). Values for xanthine, adenosine, and guanosine are from Hotchkiss, J. Biol 

 Chem. 175, 315 (1948), with a similar solvent system. 



** n-Butanol 77%, water 13%, formic acid 10% by vol.; Whatman No. 1, descending; Markham and Smith, 

 op. cit. 



' Isopropanol 85 ml., water 15 ml., coned. (28%) NHj soln. 1;3 ml.; Whatman No. 1, descending; Hershey 

 et al., J. Gen. Physiol. 36, 777 (1953). 72;? values at 20-23° detd. by the reviewer. 



/ Isopropanol 170 ml., coned. HCl (sp. gr. 1.19) 41 ml., water to make 250 ml.; Whatman No. 1, descending; 

 Wyatt, Biochem. J. 48, 584 (1951). Values redetd. at 20-23° by the reviewer. 



" Collidine 1 vol., quinoline 2 vol., mixt. satd. with 1.5 vol. water; Schleicher and Schiill No. 597 paper, 

 descending, at about 22°; Vischer and Chargaff, /. Biol. Chem. 176, 703 (1948). 



'' Isobutyric acid 400 ml., water 208 ml., 25% NHi soln. 0.4 ml.; Whatman No. 4, descending, at 22°; Lof- 

 gren, Acta Chem. Scand. 6, 1030 (1952); excepting deoxyribosides, for which solvent and conditions are as in 

 footnote e, Table II, and Rf values arecalcd. from therelative mobilities given by Tamm et al., J. Biol. Chem. 

 203, 6:3 (1953), taking the Rp oi thymidine as 0.75. 



' 5% aq. Na2HP04 satd. with isoamyl ale, both aq. and nonaq. phases being present in the trough; 

 Whatman No. 1, descending; Carter, J. Am. Chem. Soc. 72, 1466 (1950). Values for deoxyribosides are from 

 Buchanan, Nature 168, 1091 (1951). 



' Water adjusted to pH 10 with N NHiOH; Whatman No. 1, ascending, 22-23°; Levenbook, personal 

 communication, 1953. Values for methylcytosine and hydroxymethylcytosine detd. by the reviewer. 



*DR = deoxyriboside. 



252 



