ELECTROPHORETIC SEPARATION OF NUCLEIC ACID COMPONENTS 273 



TABLE II 



The Calculated Mobilities Relative to Uridylic Acid of Some Nucleotides 



AND Polynucleotides at pH 3.5 and Their Observed Movement in 0.05 M 



Ammonium Formate Buffer pH 3.5 at 20 v./cm.^" The Relative Positions 



OF These Substances on Paper Chromatograms in Solvent 1 Are Also 



Given (The Bands Are Numbered 1-6 in Order of Increasing Rf Value) . 



" A, G, C, and U denote adenylic, guanylic, cytidylie, and uridylic acids, respectively. The symbol ! is 

 used to indicate a 2',3'-monohydrogen phosphate group. 



the reverse order to that in which the mononucleotides move towards the 

 anode. At this pH the purines and pyrimidines move in the same order as 

 the nucleosides. These separations have been described by Dimroth et 

 al.,^* but the order they report for the migration of the bases at pH 3.2 is 

 not that expected theoretically, nor is it found in practice. 



" K. Dimroth, L. Jaenicke, and I. Vollbrechtshausen, Z. physiol. Chem. 289, 71 

 (1952). 



