CHAPTER 11 



Isolation and Composition of the Pentose Nucleic Acids 

 and of the Corresponding Nucleoproteins 



B. MAGASANIK 



Page 



I. Introduction 373 



II. Isolation of Pentose Nucleoproteins 374 



1. General 374 



2. Isolation of Nucleoprotein from Animal Tissues 375 



a. Fractional Centrifugation 375 



b. Extraction with 0.14 M Sodium Chloride Followed by Isoelectric 

 Precipitation 375 



c. Salt Fractionation 375 



(1) Nucleotropomyosin from Carp Muscle 376 



3. Isolation of Nucleoprotein from the Tissues of Higher Plants 376 



a. Fractional Centrifugation 376 



(1) Preparation of Cucumber Virus 4 (CV4) 377 



b. Salt Fractionation 378 



(1) Preparation of Turnip Yellow Mosaic Virus 378 



4. Isolation of Nucleoprotein from Microbial Tissues 379 



III. The Nature of Pentose Nucleoprotein 379 



IV. The Isolation of Pentose Nucleic Acids 384 



1. General 384 



2. Isolation of PNA from Animal Tissues 387 



(1) Preparation of Mammalian Tissue Ribonucleic Acid 387 



3. Isolation of PNA from the Tissues of Higher Plants 389 



(1) Isolation of PNA from Strains of Tobacco Mosaic Virus and Cu- 

 cumber Virus 389 



4. Isolation of PNA from Microbial Tissues 390 



(1) Preparation of PNA from Yeast 391 



V. The Nature of PNA 391 



VI. The Nucleotide Composition of PNA 394 



1. General Considerations 394 



2. Analytical Procedures 395 



3. PNA from Animal Tissues 396 



4. PNA from Microbial Tissues 402 



5. PNA from Plant Viruses 403 



6. Conclusions 40o 



I. Introduction 



The early history of the discovery of the nucleic acids is described in 

 Chapter 1. 



373 



