ANEURINE (thiamine) 



method. Moreover, riboflavine deficiency has a similar effect on the 

 oestrus cycle, and the presence of riboflavine in the test substance 

 would presumably interfere with aneurine assays. 



References to Section 7 



1. B. C. P. Jansen and W. F. Donath, Proc. K. Akad. Wetensch. 



Amsterdam, 1926, 29, 1390. 



2. E. S. Edie, W. H. Evans, B. Moore, G. C. E. Simpson and A. 



Webster, Biochem. J., 1912, 6, 234. 



3. H. W. Kinnersley and R. A. Peters, ihid., 1928, 22, 419 ; I933. 



27, 225, 232. 



4. K. H. Coward, J. H. Bum, H. W. Ling and B. G. E. Morgan, 



ihid., 1933, 27, 1719. 



5. A. Seidell, U.S. Publ. Health Rep., 1922, 37, 15 19. 



6. K. H. Coward and B. G. E. Morgan, Biochem. J., 1939, 38, 658. 



7. R. Passmore, R. A. Peters and H. M. Sinclair, ibid., 1933, 27, 842 ; 



R. A. Peters, H. Rydin and R. H. S. Thompson, ibid., 1935, 29, 

 53 I 1938, 32, 2031. 



8. T. H. Jukes and H. Heitman, /. Nutrition, 1940, 19, 21. 



9. H. C. Sherman and A. Spohn, /. Amer. Chem. Soc, 1923, 45, 2719. 



10. B. C. Guha and J. C. Drummond, Biochem. J., 1929, 23, 880. 



11. K. H. Coward, ibid., 1936, 30, 2012. 



12. F. W. Schultz and E. M. Knott, /. Nutrition, 1936, 12, 583. 



13. L. S. Fridericia, P. Freudenthal, S. Gudjonnsson, G. Johansen and 



N. Schoubye, /. Hygiene, 1927, 27, 70. 



14. M. I. Smith, U.S. Publ. Health Rep., 1930, 45, 116. 



15. F. F. Heyroth, Bull. Basic Science Rep., 1932, 4, i. 



16. H. W. Kinnersley, R. A. Peters and V. Reader, Biochem. J., 1930, 



24, 1820. 



17. T. W. Birch and L. J. Harris, ibid., 1934, 28, 602. 



18. K. H. Coward and B. G. E. Morgan, ibid., 1941, 35, 974. 



8. 2VUCROBIOLOGICAL ASSAY OF ANEURINE 



An entirely different method of assaying anemine preparations 

 from the foregoing are fermentation tests ; these are carried out with 

 micro-organisms for which aneurine is an essential growth factor. The 

 principle of the method is that the selected organism is grown on a 

 medium that gives optimal growth on addition of aneurine, and the 

 amoimt of growth obtained with the test solution is then compared 

 with that given by a control containing known amounts of aneurine. 

 The amount of growth is measured in some suitable way, e.g. turbi- 

 dimetrically or by the amount of carbon dioxide, lactic acid or other 

 metabolite formed. 



32 



