ANEURINE (thiamine) 



substances, and E. E. Fitzgerald and E. B. Hughes ^^ eliminated this 

 particular source of error by subtracting from the response to the test 

 solution, the response to another portion of the solution in which the 

 aneurine had been inactivated by autoclaving with sulphite. 



Some of the difficulties of the L. fermenti turbidimetric method are 

 said to be overcome by using a plate method of assay, similar in 

 principle to that used for the assay of antibiotics, except that the 

 zones formed around the holes cut in the agar are zones of stimulation 

 and not of inhibition. ^^ The method is not very sensitive, but with 

 yeast and yeast products the results are at least as accurate as those 

 obtainable by other methods. ^^ Takadiastase was used to liberate 

 the aneurine from the yeast. 



References to Section 8 



1. A. S. Schultz, L. Atkin and C. N. Frey, /. Amer. Chem. Soc, 1937, 



59, 948, 2457 ; 1938, 60, 1514. 



2. K. Heyns, Z. physiol. Chem., 1939, 258, 219. 



3. H, H. Bunzell, Ind. Eng. Chem., Anal. Ed., 1942, 14, 279. 



4. A. S. Schultz, L. Atkin and C. N. Frey, /. Biol. Chem., 1940, 136, 



713- 



5. A. S. Schultz, L. Atkin and C. N. Frey, Ind. Eng. Chem., Anal. Ed., 



1942, 14, 35. 



6. A. S. Schultz, L. Atkin, C. N. Frey and R. R. Williams, /. Amer. 



Chem. Soc, 1941, 63, 632. 



7. R. J. Williams, J. R. McMahan and R. E. Eakin, Univ. Texas 



Publ., 1941, No. 4137, 31. 



8. N. S. Scrimshaw and W. B. Stewart, /. Biol. Chem., 1944, 155, 79. 



9. H. F. Deutsch, ibid., 1944, 152, 431. 



10. H. G. Obermeyer and L. Chen, ibid., 1945, 159, 117. 



11. H. G. K. Westenbrink, E. P. S. Parv6, A. C. van den Linden and 



W. A. van den Broek, Z. Vitaminforsch., 1943, 63, 218. 



12. L. Atkin, A. S. Schultz and C. N. Frey, /. Biol. Chem., 1939, 129, 



471. 



13. H. Laser, Biochem. J., 1941, 35, 488. 



14. E. S. Josephson and R. S. Harris, Ind. Eng. Chem., Anal. Ed., 



1942, 14, 755. 



15. W. H. Schopfer, Z. Vitaminforsch., 1935, 4, 67. 



16. A. P. Meiklejohn, Biochem. J., 1937, 31, 1441- 



17. H. M. Sinclair, ibid., 1938, 32, 2185 ; 1939, 33, 1816, 2027. 



18. T. Morell, Deut. med. Woch., 1938, 64, 1722. 



19. A. P. Meiklejohn, Biochem. J., 1937, 31, 1441 ; H. M. Sinclair, 



ibid., 1938, 32, 2185; 1939, 33, 1816, 2127; E. N. Rowlands 

 and J. F. Wilkinson, Brit. Med. J., 1938, 2, 878 ; T. Morell, 

 Deut. med. Woch., 1938, 64, 1722 ; G. Guhr, Klin. Woch., 1939, 

 18, 1028 ; R. Goodhart and H. M. Sinclair, /. Biol. Chem., 1940, 

 132, II. 



36 



