METABOLISM 



administration is resorted to, the possibility of anaphylaxis must be 

 kept in mind and the dose given slowly. C. A. Mills, ^ however, suggests 

 that parenteral administration should never be used. 



References to Section 13 



1. C. L. Steinberg, Amer. J. Digest. Dis. Nutr., 1938, 5, 680. 



2. M. B. Richards, Brit. Med. J., 1945, 1, 433. 



3. L. R. Cerecedo and L. J. Vinson, Proc. Soc. Exp, Biol. Med., 1944, 



55, 139. 



4. C. Mano, Japan J. Med. Sci., IV, 1940, 12, Proc, 98. 



5. C. A. Mills, /. Amer. Med. Assoc, 1941, 116, 2101 ; 1941, 117, 1501. 



6. Z. A. Leitner, Lancet, 1943, 2, 474. 



7. M. H. Stiles, /. Allergy, 1941, 12, 507 ; L. Scheff, /. Amer. Med. 



Assoc, 1941, 117, 609 ; C. L. Laws, ibid., 176 ; W. S. Eisenstadt, 

 Minn. Med., 1942, 25, 861 ; M. M. Mitrani, /. Allergy, 1944, 

 15, 150 ; W. Stein and M. Morgenstein, Ann. Int. Med., 1944, 

 20, 826. 



8. F. Kalz, /. Invest. Dermat., 1942, 6, 135. 



9. T. J. Haley and A. M. Flesher, Science, 1946, 104, 567. 



10. H. Molitor, Proc. Fed. Amer. Soc. Exp. Biol., 1942, 1, 309. 



11. I. M. Reingold and F. R. Webb, /. Amer. Med. Assoc, 1946, 130, 



491. 



12. N. JoUifEe, ibid., 1941, 117, 1496, 1501. 



14. METABOLISM OF ANEURINE 



Both microbiological and chemical assays have been used to 

 measure the amount of aneurine in the blood and urine of normal and 

 avitaminous humans and animals. The results indicated that the 

 blood and urine concentrations were lower in vitamin B^-deficient 

 than in normal subjects, and quite early in the history of aneurine 

 therapy it was recognised that low concentrations of aneurine in both 

 body fluids supported a diagnosis of vitamin B^ deficiency. 



Urinary excretion and the Test-Dose Method of Assessing 

 Nutritional Status 



L. J. Harris and P. C. Leong ^ introduced a method of assessing 

 the nutritional status of an individual with respect to aneurine that 

 was more reliable than a simple measurement of the urinary concen- 

 tration. The concentration of aneurine in the urine before and after 

 administration of a test dose was estimated by the thiochrome 

 method ; in vitamin B^ deficiency, very little of the test dose was 

 excreted, whereas well-nourished subjects excreted a relatively large 

 proportion. , 



63 



