ANEURINE (thiamine) 



within sixty-six hours of adding aneurine. The growth of larvae fed 

 a vitamin Bi-deficient diet, supplemented by graded doses of aneurine, 

 was proportional to the amount of aneurine. This method of assay 

 has not been adopted by other workers, however. 



References to Section 21 



1. E. G. van t'Hoog, Z. Vitaminforsch., 1935, 4, 300; 1936, 6, 118 ; 



E. L. Tatum, Proc. Nat. Acad. Sci., 1939, 26, 490 ; 1941, 27» 193. 



2. W. Trager and Y. SubbaRow, Biol. Bull. Woods Hole, 1938, 76, 75 ; 



Y. SubbaRow and W. Trager, /, Gen. Physiol., 1940, 23, 561. 



3. H. E. Martin and L. Hare, Biol. Bull. Woods Hole, 1942, 88, 428. 



4. G. Frobrich, Z. vergl. Physiol., 1939, 27, 335 ; K. Offhaus, ibid., 



384. 



5. G. Fraenkel and M. Blewett, Nature, 1943, 151, 703; 1943, 162, 506; 



Biochem. J., 1943, 37, 686 ; Proc. Roy. Soc. B., 1944, 132, 212. 



6. V. B. Wigglesworth, Parasitology, 1929, 21, 288 ; P. Buchner, 



Tiere und Pflanzen Symbiose, Berlin, 1930 ; M. Aschner, 

 Z. Morph. Okol. Tiere, 1931, 20, 368 ; A. Koch, Biol. Zbl., 1933, 

 63, 199 ; Verh. dtsch. Zool. Ges., 1933, 36, 143. 



7. L. Golberg, B. de Meillon and M. Lavoipierre, /. Exp. Biol., 1945, 



21, 84, 90. 



8. P. S. Sarma and K. Bhagvat, Current Sci., 1942, 11, 331 ; P. S. 



Sarma, G. B. L. Swami and M. Sreenivasaya, ibid., 332 ; P. S. 

 Sarma, Indian J. Med. Res., 1943, 31, 173. 



22. ANALOGUES OF ANEURINE 

 Thiazole and Pyrimidine Compounds 



In the course of the researches that led to the synthesis of aneurine, 

 a mmiber of compounds closely related to the vitamin were prepared, 

 and their biological activities were determined. Subsequently, 

 deliberate attempts were made to modify the aneurine molecule with 

 a view to ascertaining the effect of such changes on the biological 

 activity. 



As already mentioned above, Todd et al.^ prepared a substituted 

 3-pyrimidyl-thiazolium salt (see page 17) isomeric with aneurine, but 

 differing from it in the absence of a methylene group joining the two 

 rings, and in the orientation of the groups on the pyrimidine ring ; it 

 was inactive when tested on vitamin Bi-deficient rats. It also failed 

 to yield thiochrome on oxidation with potassium ferricyanide, but it 

 gave a positive formaldehyde-azo test. 



In a later paper, A. R. Todd and F. Bergel ^ described the pre- 

 paration of several compounds differing from aneurine only in the 

 nature of the substituents on the two rings. None of these analogues 



116 



