ANIMAL AND HUMAN REQUIREMENTS 



per kg. of bodyweight per day/^ and a horse 44 fig. per kg. of body- 

 weight per day.^^ In mild cases, the symptoms of riboflavine de- 

 ficiency in rhesus monkeys were relieved by the administration of 

 50 fjLg. of riboflavine per day for two to three weeks, but severe cases 

 required 100 to 500 /xg. daily.^^ The minimum daily intake necessary 

 to maintain normal health was estimated to be 25 to 30 /xg. per kg. 

 of bodyweight. Thus, the requirements of all species of mammals 

 for which data are available, including man, are of the same order 

 when calculated per kg. of bodyweight. 



Requirements of Birds and Fish 



To prevent the development of " curled-toe paralysis " in chickens, 

 the diet should contain 3-6 /xg. of riboflavine per g.^^ Ducklings 

 required a similar amount for proper development. ^^ For satisfactory 

 egg production, the diet of hens should contain at least 2 /xg. of ribo- 

 flavine per g. With a diet containing 27 /xg. per g., hens kept in pens 

 laid eggs containing 24 % of the ingested riboflavine, whilst hens kept 

 in batteries and fed a diet containing 3-3 /xg. per g. laid eggs containing 

 30 % of the ingested riboflavine.^*^ 



Young rainbow trout required 5 to 15 /xg. of riboflavine per g. of 

 diet. 21 



References to Section 13 



1. W. H. Sebrell, R. E. Butler, J. G. Wooley and H. Isbell. U.S. 



Puhl. Health Rep., 1941 56, 510. 



2. R. D. Williams, H. L. Mason. P. L. Cusick and R. M. Wilder. 



/. Nutrition, 1943, 26, 361. 



3. W. Brewer, T. Porter, B. Ingalls and M, A. Ohlson, ihid., 1946, 



32, 583. 

 3fl. M. K. Horwitt, O. W. Hills, C. C. Harvey, E. Liebert and D. L. 

 Steinberg, ihid., 1949, 39, 357. 



4. T. F. Macrae, E, C. Barton-Wright and A. M. Copping, Biochem. J., 



1944, 38, 132. 



5. H. Oldham, F. Johnston, S. C. Kleiger and H. H. Arismendi, /. 



Nutrition, 1944, 27, 435. 



6. D. R. Hagedorn, E. D. Kyhos, O. A. Germek and E. L. Sevringhaus, 



ihid., 1945, 29, 179- 

 6a. S. E. Snyderman, K. C. Ketron, H. B. Burch, O. H. Lowry, 

 O. A. Bessey, L. P. Guy and L. E. Holt, ihid., 1949, 39, 219. 



7. J. C. Drummond, " Nutritional Requirements of Man in the Light 



of Wartime Experience ", Royal Institute of Chemistry, 1948. 



8. " Food Consumption Levels in the United Kingdom ", Cmd. 7203, 



H.M.S.O., 1947. 



9. L. N. Ellis, A. Zmachinsky and H. C. Sherman, /. Nutrition, 1943, 



26, 153- 



10. G. J. Mannering and C. A. Elvehjem, ibid., 1944, 28, 157. 



11. Le R. Voris and H. P. Moore, ihid., 1943, 25, 7. 



i8q 



