EFFECT ON ANIMALS AND MAN 



Other Biological Properties 



Rats fed rations containing thyroid-active materials required a 

 factor present in liver, fish solubles and tomatoes.^^ The factor was 

 present in anti-pernicious anaemia active fractions but, whereas 

 J. J. Betheil and H. A. Lardy ^^ and G. A. Emerson ^^ found vitamin 

 Bi2 to be active, B. H. Ershoff ^^ found it to be ineffective. Incon- 

 sistent results were obtained when attempts were made to use hyper- 

 thyroid rats for the assay of vitamin Bjj (page 535). 



Vitamin B^g did not produce a response in cobalt-deficient lambs 

 either after injection of 125 /itg. or after being fed orally for 6 weeks. ^' 



Crystalline vitamin B^g prevented gizzard erosion in chicks. ^^ 



Vitamin B^g, like pteroylglutamic acid, increased the incidence and 

 size of Rous tumour implants in chicks, and the effect of the two 

 substances given together was greater than that of either alone. ^^ 



References to Section 5 



1. R. West, Science, 1948, 107, 398. 



2. B. E. Hall and D. C. Campbell, /. Lab. Clin. Med., 1948, 33, 1646 ; 



C. C. Ungley, Lancet, 1948, 1, 771 ; Brit. Med. J., 1948, 2, 154. 



3. C. C. Ungley, ihid., 1949, 2, 1370. 



4. J. C. Patel, ihid., 1948, 2, 934 ; T. D. Spies, R. E. Stone and 



T. Aramburu, Southern Med. J., 1948, 41, 487, 522 ; T. D. Spies, 

 G. G. Lopez, F. Milanes, R. L. Toca and B. Culver, ihid., 523 ; 

 L. Berk, D. Denny-Brown, M. Finland and W. B. Castle, New 

 England J. Med., 1948, 239, 328 ; T. D. Spies, R. E. Stone^ 

 G. G. Lopez, F. Milanes, T. Aramburu and R, L. Toca, Postgrad. 

 Med., 1948. 4, 89 ; T. D. Spies, R. M. Suarez, G. G. Lopez, 

 F. Milanes, R. E. Stone, R. L. Toca, T. Aramburu and S. Kartus, 

 /. Amer. Med. Assoc, 1949, 139, 521 ; T. D. Spies and 

 R. M. Suarez, Blood, 1948, 3, 1213. 



5. R. E. Stone and T. D. Spies, /. Lah. Clin. Med., 1948, 33, 1019 ; 



J. F. Schieve and R. W. Rundles, ihid., 1949, 34, 439, 



6. D. M. Dunlop and W. M. Wilson, Lancet, 1949, 1, 754 ; C. M. 



Miller and E. H. Moorhouse, Brit. Med. J., 1949, 2, 151 1. 



7. H. Lichtman, J. Watson, V. Ginsberg, J. V. Pierce, E. L. R. 



Stokstad and T. H. Jukes, Proc. Soc. Exp. Biol. Med., 1949, 72, 



643. 



8. E. H. Morgan, E. E. Hall and D. C. Campbell. Proc. Staff Meetings 



Mayo Clinic, 1949, 24, 594. 



9. K. Hausmann, Lancet, 1949, 2, 962. 



10. T. D. Spies, R. E. Stone, G. G. Lopez, F. Milanes, R. L. Toca and 



T. Aramburu, ihid., 1454. 



11. F. H. Gardner, J. W. Harris, R. F. Schilling and W. B. Castle, 



/. Lah. Clin. Med., 1949, 34, 1502. 



12. F. H. Betheil, M. C. Meyers and R. B. Neligh, ihid., 1948, 33, 1477. 



