MISCELLANEOUS WATER-SOLUBLE GROWTH FACTORS 



3. S. J. FoUey, E. W. Ikin, S. K. Kon and H. M. S. Watson, Biochem. 



J., 1938. 32, 1988. 



4. E. L. R. Stokstad and P. D. V. Manning, /. Biol. Chem., 1938, 



125, 687. 



5. E. L. R. Stokstad, P. D. V. Manning and R. E. Rogers, ibid., 1940, 



132, 463. 



6. D. V. Frost and C. A. Elvehjem, ibid., 1937, 119» xxxiv. 



7. H. Kringstad and G. Lunde, Z. physiol. Chem., 1939, 261, no; 



G. Lunde and H. Kringstad, Naturwiss., 1940, 28, 157 ; /. 

 Nutrition, 1940, 19, 321, 



8. A. E. Schumacher, G. F. Heuser and L. C. Norris, /. Biol. Chem., 



1940. 135, 313. 



9. F. W. Hill, L. C. Norris and G. F. Heuser, /. Nutrition, 1944, 28, 



175. 



10. M. L. Scott, L. C. Norris and G. F. Heuser, /. Biol. Chem., 1947, 



167, 261. 



11. H. Sprince and D. W. Woolley, /. Exp. Med., 1944, 80, 213. 



12. H. Sprince and D. W. Woolley, /. Amer. Chem. Soc, 1945, 67, 



1734- 



13. D. W. Woolley and H. Sprince, Fed. Proc, 1945, 4, 164. 



14. D. W. Woolley, /. Biol. Chem., 1945, 159, 753. 



15. D. W. Woolley, ibid., 1946, 162, 383. 



16. D. W. Woolley, ibid., 1946, 166, 783 ; 1948, 172, 71. 



17. P. A. Plattner and N. Clauson-Kaas, Helv. Chim. Acta, 1944, 28, 



188. 



18. W. A. Krehl and J. S. Fruton, /. Biol. Chem., 1948, 173, 479. 



19. D. W. Woolley, ibid., 1949, 176, 1291. 



20. D. W. Woolley, ibid., 1299. 



21. D. W. Woolley, ibid., 1947, 171, 443. 



22. G. Fraenkel, M. Blewett and M. Coles, Nature, 1948, 161, 981 ; G. 



Fraenkel, Brit. J. Nutrition, 1948, 2, i. 



6. MISCELLANEOUS GROWTH FACTORS FOR ANIMALS 

 Grass Juice Factor 



The " grass juice factor " is a relatively labile vitamin, said by 

 Kohler et al} to be necessary for normal growth in rats and guinea- 

 pigs. It was obtained from grass juice by shaking with, a mixture of 

 chloroform and amyl alcohol, removing proteins and then treating 

 with charcoal. The activity was present in the filtrate. 



A substance apparently identical with it was reported by M. D. 

 Cannon and G. A. Emerson, 2 who found that guinea-pigs failed to 

 thrive on a highly purified diet, supplemented by vitamins in amounts 

 adequate for rats, unless either lettuce or grass was given. The factor 

 was water-soluble and stable at 100° C. for one hour. The same 

 factor was isolated from the fraction of an aqueous liver extract that 



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