508 I. iKEDA : 



in our knowlodo'e of tliis interei^tino' animal, tlio investia;ation, 

 of wUieli an account is given in the followiui;- pages, was under- 

 taken, and tliongli the residts are far from exhaustive, I liope 

 they will hel]) to advance our knowledge of the sul^jeet. 



^[v studv was hegun in tlie sunnner of LSDS during a stav 

 at the ]\risaki JMarine Biological Station and later was continued 

 at that Station as well as in the Zoological Institute of the Science 

 College. 



At Al)uratsubo, a small inlet close to the Station, is found 

 a species <>f FJioronix, which has been named hv Dr. ()ka ('97) 

 r. ijimai.''' Its colonies adhere to the overhanging ledges of rocks 

 near the shore. As the water at the place is always cahn and at 

 low tides recedes so as to almost expose the ledges, the animals 

 can be easily collected. During the greater j^art of the year, eggs 

 and young embryos, clustered together, in what may conveniently 

 be called ernhryonal 'masses, are found adhering to the lophoplioral 

 crown of the adult, one on each side of the median line. These 

 furnished materials for the study of fertilization, segmentation and 

 the earlv larval stashes. The larva' in the Actinotrocha stage are 

 found swimming in the inlet and are caught with the surface net. 

 As will later be fully described, there occur four kinds of the 

 larvce, which ]io doubt represent as many species, including the 

 common Phoronis ijimai. 



The specimens, both adult and larval, were killed with the 

 saturated solution of corrosive sublimate in l^o acetic acid or with 

 Flemming's fluid. Of the various colouring methods tried on the 

 sections, double-staining with eosin or safranin and Delatleld's 

 hcTmatoxylin gave the most satisfactory results. 



*For a discussion of the status of this species, see Supplementary Nofes. 



