THE BEGINNINGS OF LIFE. 



APPENDIX C. 



Comparative Experiments. 



In the following experiments, each fluid (unless a state- 

 ment is made to the contrary) was boiled continuously for ten 

 minutes, after having been placed in its flask. Then, with 

 the neck either open, sealed, or plugged, the bulb of the 

 flask was immersed in a water-bath maintained at a tem- 

 perature of 8o°-95° Fahr., during both day and night \ 



First Set of Experiments (I — XV). 

 a. Fluid exposed to Air in a Flask with a short Open Neck. 



No. I. Urine in twenty-four hours was still clear and 

 free from deposit. In forty-four hours the fluid was very 

 slightly turbid, and on microscopical examination Bacteria 

 and TorulcB were found, though not in very great abundance. 

 In sixty-eight hours the fluid was decidedly turbid. 



No. II. Hay Infusion in twenty-four hours was still 

 clear. In forty-four hours the fluid was very turbid, and a 

 drop, on examination, showed multitudes of Bacteria of 

 diff"erent kinds, exhibiting languid movements. In sixty- 

 eight hours the turbidity had become much more marked, 

 and there was also a certain amount of sediment. 



* When infusions have been employed, they have all been made as 

 strong as possible, and have been filtered before use. Warm water has 

 been added in quantity just sufficient to cover the substance which 

 was to be infused ^this being cut into very small pieces), an the 

 mixture has then been kept at a temperature of from i io°-i3o°Fahr. for 

 three or four hours. 



