APPENDIX C. xxxui 



d. Fluid in a Flask having a Neck two feet long, beiit at right 



angles shortly above the bulk, and provided with a firm 

 Plug of Cotton-Wool twelve i?iches in length. 



No. X. Urine remained quite bright and clear during 

 the fifteen days in which it was kept under observation in the 

 water-bath ^. 



No. XL Hay Infusion showed a very slight amount of 

 sediment after forty-four hours, which seemed to increase 

 somewhat during the next three days. The fluid afterwards 

 appeared to undergo no further change, though it remained 

 in the warm water-bath for fifteen days \ 



No. XII. Turnip Infusion in four days showed a well- 

 marked turbidity, and also very many flakes of a broken 

 pellicle ^. 



e. Fluid (in vacuo) i?t a Flask, the Neck of which was her- 



metically Sealed by means of the Blowpipe Flame during 

 Ebullition. 



No. XIII. Urine in forty-four hours showed a very 

 slight amount of sediment. During the next two days the 

 sediment very slightly increased, but was still small in amount. 

 At the expiration of fifteen days, no further increase in the 

 turbidity having taken place, the fluid was examined. The 

 vacuum was still partially preserved, as evidenced by the 

 rapid inbending of a portion of the neck of the flask after it 

 had been carefully made red-hot. When opened, the odour 

 of the fluid was stale, but not foetid, and its reaction was still 

 faintly acid. On microscopical examination Bacteria and 

 TorulcB were found in tolerable abundance. 



going any apparent change, and then only became turbid under the 

 influence of a higher temperature. Yet some of the same fluid, in a 

 flask which was hermetically sealed during the first ebullition (No. XV.) 

 behaved as such an infusion usually does, and became quite turbid in 

 forty-eight hours. 



^ Flask still in my possession, unopened. 

 l^OL. II. C 



