APPENDIX C. 



microscopically, it was found to contain multitudes of very 

 languid Bacteria. 



No. XVII. Neutralized Infusion of Turnip + \ gr. 

 of Cheese ^ in thirty-six hours showed a well-marked 

 pellicle^. When the flask was opened, after seventy-two 

 hours, there was a violent outrush of gas, though the fluid 

 was still neutral. Portions of the thick pellicle were found, 

 on microscopical examination, to be made up of Bacteria, 

 Vihriones, and an abundance of long, interlaced Leptothrix 

 filaments. Bacteria also existed abundantly in the fluid, 

 though their movements were very languid. 



c. Fluid in a Bent-neck Flask, having Fight acute Flexures. 



No. XVIII. Simple Turnip Infusion after forty-eight 

 hours showed no change. It was kept in the warm-bath 

 for twelve days, and during the whole of this time the fluid 

 remained quite clear. The tube was then broken i^ inch 

 above the bulb (which was re-immersed in the bath),, leaving 

 the fluid exposed to the air through the straight open tube. 

 The fluid at this time was odourless, and its re-action was 

 still faintly acid. 



The infusion remained thus exposed for ^ix days without 

 undergoing any apparent change. On the eighth day a very 

 slight whitish sediment was noticed, which had increased in 

 quantity by the tenth day, though there was still no trace of 

 general turbidity. .On the eleventh day som.e of the sediment 

 was examined in a drop of the fluid, and it was found to be 

 wholly composed of rather large Torulce cells — the largest 

 being about 3 oVo'' i^^ diameter, thoilgh all the smaller sizes 

 were abundantly represented. Not a single Bacterium or 



^ The filtered infusion of turnip was neutralized by liquor potassae. 

 The cheese (^Cheddar) was new and not in the least mouldy. 



^ The fluid itself being somewhat opaque, the first stages of increased 

 turbidity from presence of Bacteria could not be detected. 



