APPENDIX C. xxxvii 



No. XXL Neutral Turnip Infusion + ^ gr, of 

 Cheese, showed a well-marked pellicle on its surface in 

 twenty-four hours. In thirty-six hours the first pellicle had, 

 in great part, sunk to the bottom of the flask, though its place 

 on the surface was already taken by a new, though thin, 

 scum-like layer. After seventy-two hours, the flask was 

 opened ; there was 7to foetid odour of the fluid, and its re- 

 action was still neutral. Examined microscopically the fluid 

 showed an abundance of Bacteria, and also of short monilated 

 filaments. There were, however, none of the ordinary kind 

 of Vibriones, and no Leptothrix. All the Bacteria exhibited 

 very languid movements. 



Third Set of Experiments (XXII — XXX). 

 a. Fluid exposed to Air i?i a Flask ivith a short Open Neck. 



No. XXII. Urine in twenty-four hours show^ed no 

 change ; though in forty-six hours the turbidity was well 

 marked ^. Examined microscopically it was found to con- 

 tain an abundance of Bacteria. 



b. Fluid in contact with Ordinary Air and its. Particles ; 

 Neck of Flask Sealed after the Fluid had become Cold. 



No. XXIII. Urine in eighteen hours showed a distinct 

 pellicle, though there was not much general turbidity. 

 During the next few days the old pellicle fell to the bottom, 

 and a new one formed. 



c. Fluid in a Bent-neck Flask, having Eight acute Flexures. 

 No. XXIV. Urine in forty-eight hours showed no 

 change. After twelve days there was still no general tur- 

 bidity, though there was a slight flocculent deposit of an un- 



1 Some of the same fluid, exposed in a similar flask, without previous 

 boiling, became turbid in eight hours, and lighter in colour ; whilst, 

 after twenty hours, the turbidity was extremely well marked. 



