APPENDIX C. xliii 



and, in addition, a small amount of deposit. On examina- 

 tion, the fluid and the pellicle were found to contain an 

 abundance of Bacteria, Vibriones, and Leptothi'ix filaments. 



No. XXXIX. Turnip Infusion + ^V of Carbolic Acid, 

 after eight days showed no appreciable alteration in appear- 

 ance ^ no trace of pellicle or deposit. When examined 

 microscopically, however, the fluid was found to contain 

 some very minute Bacteria, though they were by no means 

 abundant. 



No. XL. Hay Infusion had become quite turbid in 

 twenty-four hours, and several shades lighter in colour. 

 After forty-eight hours the colour of the infusion was still 

 lighter ; there was more turbidity, and some sediment. On 

 microscopical examination, the fluid was found to contain 

 an abundance of Bacteria, Vibriones, and short Leptothrix 

 filaments. 



No. XLI. Hay Infusion -i- ^v of Carbolic Acid, 

 showed no apparent change^ after forty-eight hours, and 

 when examined microscopically it revealed no trace of 

 Bacteria, or other organisms. The neck of the flask was 

 then again closed. On the twelfth day the fluid had still 

 undergone no change in appearance, and when examined 

 microscopically it still showed no trace of organisms, though 

 the fluid was — as it had been at the time of the first exami- 

 nation — full of minute, undissolved particles of carbolic acid. 



Fluids boiled for five minutes, and half-filling hermetically 

 Sealed Flasks containing Ordinary Air. 



No. XLII. Hay Infusion, after forty-eight hours, showed 

 no change, and continued to remain quite clear and free 



^ It had been rendered turbid from the first, by the carbolic acid. 

 '^ The fluid had been rendered paler and turbid fi-om the first, by the 

 addition of the carbolic acid. 



