APPENDIX C. xlv 



No. XLV. Turnip Infusion -i- ^^ part of Carbolic 

 Acid, showed no increase of turbidity^ for the thirteen days 

 during which it was kept under observation. Before the 

 flask was opened it was ascertained that the vacuum was w^ell 

 preserved. The odour of the fluid was unaltered, and on 

 microscopical examination no Bacteria^ or other living 

 things, were found ^. 



No. XLVI. Hay Infusion, after forty-eight hours, 

 showed no change, though, in seventy-two hours, there was 

 perceptible a very small amount of a dirty greyish deposit. 

 By the fifth day the deposit had slightly increased, and on the 

 seventh day there was a trace of turbidity in the fluid. It 

 did not undergo much further change, so that, on the twelfth 

 day, the flask was opened. The vacuum was found to have 

 been very slightly impaired; the odour of the fluid was 

 almost natural, and its re-action was slightly acid. On micro- 

 scopical examination of the deposit, Bacteria^ Vtbriones, short 

 Leptothrix filaments, and TorulcB, were found, though not in 

 very great abundance. 



No. XL VII. Hay Infusion + ^V P^^* of CarboLLc 

 Acid, showed no apparent change for the first four days. On 

 the fifth day there was a smaU quantity of pow^der-like 

 sediment, and one dirty greyish-coloured flake. On the 

 seventh day there were more small flakes at the bottom, and a 

 slight general turbidity of the fluid. On the twelfth day, the 

 turbidity and deposit having increased, the flask was opened 

 — after it had been first ascertained that the vacuum had only 

 been slightly impaired. The re-action of the fluid was still 

 strongly acid. On microscopical examination of some of the 

 deposit, there was found, amongst granular flakes and aggre- 

 gations, a large number of 2'orulte cells, of most various 



^ This fluid was whitish, and somewhat opaque, from the first. 

 "^ For other experiments showing a similar sterility, induced by a slight 

 acidification with acetic acid, see ' Nature,' 1870, No. 37, pp. 226, 227. 



