150 



THE ACTINOMYCETES, VOL. Ill 



6. Basic substance soluble in water at acid pH, phagolessin 

 insoluble in chloroform 



7. Neutral or weakly basic compound, soluble in phagostatin 

 chloroform 



8. Benzenoid-type compound containing no ni- ramnacin 

 trogen 



9. Contains nitrogen, water-soluble substance desertomycin 



totomycin 



10. Xo data to permit classification miramycin 



KEY NO. 3 



Aiilihiotics Wliicli Have Anlifiiiifial Activity' 



I. Antibiotics active not only against fungi but also against gram-positive bacteria 



A. Active against il/. tuberculosis var. hominis but not against other bacteria 



1. Weakly basic substance, light-absorption max- toyocamycin 

 ima in water at 230 and 277, and in 0.1 to unamycin B 

 0.05 X HCl at 235 to 236 and 273 



2. Oil, light-absorption maximum at 2:55 in hygroscopin A 

 ethanol 



B. Acti^'e against strains of Nocardia asteroides, but not against other bacteria or 

 streptomycetes 



1. Light-absorption maxima at 203 and 268 solu- flavofungin 

 l)le with difhculty in water 



2. Basic, water-soluble substance eulicin 



C. Active against strains of Nocardia and Sfreptomijccs, but not against bacteria 



1. Light-absorption maximum at 302 in meth- eum3^cetin 

 anol 



D. Active against gram-positive bacteria sfricto scnsu 



1. Substances for which one maximum of light al)- 

 sorption has been reported, as follows: 



a. 230 in methanol 



b. 240, with shoulder at 255 to 270; yellow, 

 water-insoluble substance 



c. 270 in methanol; yellow substance 



d. 275 in methanol 



e. 304 in ethanol 



f. 310, with strong end-absorption below 250 br,vamycin 

 in 6 N H2SO4 ; sulfur-containing polypep- 

 tide 



2. Substances for which two maxima of light ab- 

 sorption have l)cen reported 



a. 215 and 257 to 258 in methanol mycospocidin 



b. 230 and 285 in water (luestiomycin B 



c. 240 and 267 to 268 in ethanol or methanol azalomycin F 



musarin 



melanosporin 

 lu'grostatin 



xanthicin 

 antibiotic F 256 

 seligocidin 



In this key, actinomycetes, including mycobacteria, are considered as gram-positive bacteria. 



