DESCRIPTIONS OF ANTIBIOTICS 



265 



not be used because of sensitivity of tlie patient 

 or resistance of the orji;anisni. Amoebiasis. 

 References: 



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therapy 2: 693-696, 1952. 



3. Haight, T. H. and Finland, M. Proc. Soc. 



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4. Haight, T. H. and Finland, M. Proc. Soc. 



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13. Bimch, R. L. and McGuire, J. M. U. S. 



Patent 2,653,899, September 29, 1953. 



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Ann. Meeting U. S. Livestock Sanitary 

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Kryllironi_> oiii IJ 



I'roditccd by: Sireploiui/ce.s ert/threii.s. 



Synorif/in: Closely related to erythromycin. 



Remarks: The i)roportions of the erythromycins 

 jjroducetl can be varied by changing the nitrogen 

 content of the culture media (5). 



Method of extraction: I. Broth at pH 9.5 ex- 

 tracted with chloroform or amyl acetate, then 

 l)ack-extracted into 0.1 M phosphate buffer at pH 

 5.2. Extraction procedures repeated. Purified and 

 separated from erythromycin by column chro- 

 matography on powdered cellulose with 0.01 N 

 XH4OH, saturated with methyl isobutyl ketone 

 as develo]3er and eluant, or l>y countercurrent 

 distribution (acetone-methyl isobutyl ketone-0.1 

 A' i)hosphate buffer, pH 6.5, 1:20:20). Active frac- 

 tions concentrated in vacuo; extracted from the 

 aqueous solution with chloroform at pH 9.6 to 

 10.5. Chloroform evaporated oft", residue extracted 

 with ether, again eva])orated, and the antibiotic 

 crystallized from acetone (1,2). II. Amyl acetate 

 extract of broth back-extracted into aqueous 

 acetic acid at pH 5.0 to 6.5 and traces of amyl 



