DESCRIPTIONS OF ANTIBIOTICS 



•2\): 



activity on Voshida sarcoma aiul ascites hepa- 

 toma (12, 15). 



To.riciti/: Mice tolerate 6 mg per kg suhcutane- 

 ously or intravenously, and 108 mg per kg orally 



References: 



1. Hata, T. et al. Kitasato Arch. K.xptl. Med. 



22: 229-242, 19-49. 



2. Hata, T. et al. J. Antibiotics (Japan) 3: 



313-325, 1950. 



3. Kiiroya, M. et al. J. Antibiotics (Japan) 



4: 363-366, 1951. 



4. Hosoya, S. et al. Japan. J. I^.xptl. Med. 



21: 411-417. 1951. 



5. Ho.soya, S. et al. Japan. J. I^\i>tl. Med. 



22: 313-316, 1952. 



6. Hata, T. et al. J. Antil)iotics (Japan) 5: 



529-534, 1952. 



7. Sano, Y. J. Antibiotics (Japan) 5: 535- 



538, 1952. 



8. Nakase, Y. and Hata. T. J. Antiliiotics 



(Japan) 5: 542-547, 1952. 



9. Hata, T. et al. Kitasato Arch, l-^xptl. Med. 



24: 447-457, 1952. 



10. Sano, H. Kitasato Arch. Exptl. Med. 24: 



459-467, 1952. 



11. Nakase, Y. and Hata, T. Kitasato Arch. 



Exptl. Med. 24: 469-480, 1952. 



12. Hosoya, S. et al . J. Antil)iotics (Japan) 



6.\: 42, 1953. 



13. Osato, T. et al. J. Antil)iotics (Japan) 



6A: 52-56, 1953. 



14. Kamada, T. J. Antibiotics (Jajnin) 6A : 



172-181, 1953. 



15. Koga, F. J. Antibiotics (Japan) TV: 176, 



1954. 



16. Govorcin, B. Tehnicki Pregled Zagreb 



8: 43 52, 1956. 



.^l;iiiii«)si«l«>slfeptoniyciii 



Produced hi/: Streptoitu/ces griseiis (1). This 

 organism also produces c.ycloheximide, strei)tocin, 

 and streptomycin (6). 



Si/nont/iii: Streptomycin B (1). 



Remarks: Mannosidostreptomycin is cleaved to 

 streptomycin by the enzyme mannosidostrejito- 

 mycinase (5). 



Method of e.r traction: I'^xtracted from bi-(jtli 

 along with streptomycin. Separated by chroma- 

 tography on acid washed alumina with 50 per 

 cent methanol as solvent and developer. The 

 more firmly absorbed, less active fractions con- 

 tain mannosidostreptomycin. Crystallized as the 

 reineckate and converted to the hydrochloride 

 (1, 2). See alsf) II and \'I under streptomycin. 



Chemical and physical properties: Trihi/dro- 

 chloride: White amorphous powder; m.p. 179- 

 182°C (decomposition) (1) or isotropic hexagonal 

 plates or prisms (dihydrate). [a]^ = —54.1° 

 (c = 1 per cent in water). pK'^, = 7.6. Anht/droiis 

 form: C = 38.25%; H = 6.22%; N = 11.31%; 

 CI = 12.52%. Co7H490nN7-3HCl (1, 2, 4). Tn- 

 reineckate: Large thin plates containing 8.10 per 

 cent water of crystallization. Anhydrous form: 

 m.p. 178-179°C (decomposition, corrected). Struc- 

 tural formula (7) : 



CHjOH 



OH 



