38 



NATURE, FORMATION, AND ACTIVITIES 



Table 13 



Composition of Jour media found very suitable for 



production of antibiotics by actinomycetes 



(Warren et al., 1955) 



Media components 



Soyl)ean meal FF grits, 

 ediV:ile grade 



Peptone, technical grade. 



Corn steep (liquid) 



Glucose, technical grade. 



Molasses, Brer Rabbit 

 (green label) 



Dextrin 



Curbay, B. G 



Glycerol 



NaCl 



CaCOg 



K0HPO4 



Media numbers and compo- 

 sition 



A-4 A-4h A-9 A-12 



10.0 

 20.0 



10.0 



5.0 

 2.0 

 2.0 



nine, histidine, and leucine) to an autoclaved 

 aqueous extract of soybeans increased con- 

 siderably the yield of streptomycin. The 

 addition of monoamino acids (glutamic, 

 aspartic) was found to be unfavorable for 

 both growth and streptomycin production 

 (Kazanskaia and Andreeva, 1959). 



The various constituents of production 

 media are selected on the basis not only of 

 their value in permitting the elaboration of 

 the antibiotics, but also of their costs. Soya 

 bean and corn products have l)een important 

 in this respect. 



Warren and coworkers (1955) showed that 

 the four media listed in Table 13 permitted 

 the production of a large variety of anti- 

 biotics by actinomycetes. They concluded 

 that the production of most antibiotics of 

 actinomycetes should occur at a detectable 

 level in at least one of them. 



Table 14 



Influence of nitrogen source on composition of actinomycin complex 



(Wak.sman et al., 1958) 



Table 15 



Influence of amino acids on actinomycin synthesis 

 (Katz and Goss, 1958) 



* Denotes an unidentified component which moves faster than Component V in circular i^aper chro- 

 matography. 



