146 



THE ACTINOMYCETES, VOL. Ill 



(1) pH indicator; blue at alkaline pH and 

 red at acid pH 



(2) Indicator; yellow at acid pH and violet 

 at alkaline pH 



(3) Ba.sic polypeptide 



c. Essentially water-insoluble substances 



(1) Violet substances 



(2) Reddish substances 



(3) Yellow substances 



(4) Colorless or white substances 

 (a) Acidic substances 



(b) Neutral substance 



(c) Basic substance 



(d) Polypeptides 



(e) Miscellaneous compounds, soluble 

 in chloroform 



(f) Miscellaneous compounds, insoluble 

 in chloroform 



(g) Solubility in chloroform not known 



(5) Color unknown 



(a) Basic compoiuid 



(b) Acidic compound 



(c) Antibiotic soluble in chloroform 

 d. Miscellaneous compounds 



(1) Mixture of lytic enzymes 



(2) Toxic pigment 



(3) Bright yellow pigment 



coelicolorin 



vinacetin 



actinoidin 



mycetin 

 antibiotic 1212 

 microcins 

 pluramycin B 

 actiduins 

 antibiotic K 125a 

 vinacetin 

 zaomycin 



antibiotic X 206 

 antibiotic X 464 

 aspartocin 

 nigericin 

 longisporin 

 mesenterin 

 aspartocin 

 phytoactin 

 antibiotic F 416 

 antibiotic J 4 

 exfoliatin 

 griseomycin 

 lustericin 

 sulfactins 

 griseoflavin 

 primycin 



antibiotic E 129 (component 

 O) 



monamycin 



cardicin 



phalamycin 



actinomycctin 

 bacteriolytic factors 

 antivirubin 

 chromomycin A3 



KEY NO. 2 



Antibiolics Which Are Active against Gram-positive and Gram-negative Bacteria 



A. Antibiotics for which one maximum of light absorption has been reported 

 1. 218.5 antibiotic PA 132 



