496 PFEIFFERELLA, AND CERTAIN ALLIED ORGANISMS 



Resistance.- — Not known. 



Metabolism. — Aerobe, facultative anaerobe. Optimum temperature for growth 37° C, 

 but growth occurs slowly at 20° C. No haemolysis of sheep's or horse's red cor- 

 puscles. Old cultures have a tendency to become brownish in colour. Foetid odour, 

 especially noticeable in broth or serum broth cultures, and in corked agar cultures. 



Biochemical. — Acid and gas in glucose only. L.M. slight acid. Indole -\- ; M.R. — ; 

 V.P. — ; nitrates reduced ; HjS -|- ; NH3-] — f- ; M.B. reduced; catalase-j — \-. 



Antigenic Structure. — Appears to be antigenically homogeneous, as tested by agglutina- 

 tion and complement fixation, but not many strains have been tested. 



Pathogenicity. — Pathogenic for guinea-pigs, rabbits, and mice. After intravenous injection 

 of rabbits a mucopurulent, sometimes hsemorrhagic, nasal discharge develops, 

 containing large numbers of the bacilli. Chronic rhinitis may develop, with atrophy 

 of the turbinate bones. Death occurs in from 24 hours to several weeks, according 

 to the dose injected. Intraperitoneal injection of 0-25 ml. of a saline suspension 

 off agar into guinea-pigs is fatal in 12 hours ; post mortem, there is peritonitis 

 with abundant haemorrhagic exudate. A similar dose injected subcutaneously 

 into mice is fatal in 48 hours ; post mortem, there is gelatinous oedema at local 

 site, and enlargement of the spleen. Neither in guinea-pigs nor in mice can the 

 bacillus be recovered from the blood. 



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