PASTEURELLA PSEUDOTUBERCULOSIS 783 



forms show great irregularity of staining ; the barred and granular type of staining 

 is very common. Gram-negative, and non-acid-fast. 

 Agar Plate.— 2i hours at 31° C. Round, 0-5-1 -0 mm. in diameter, umbonate, granular, 

 translucent, greyish-yellow colonies, with dull, finely granular or beaten- 

 copper surface and entire edge ; butyrous consistency ; easily emulsifiable ; differ- 

 entiated into a raised, more opaque centre and a flat, clearer periphery with radial 

 striation. A rough variant 



with an irregular surface and ^ • . ' * * . i 



a crenated edge also occurs. •- » * ' % * 



Deep Agar Shake. — 5 days at 37° C. "• .» , " . / * ' 



Heavy surface growth. No * * .V • 



colonies beneath surface. , ' % \ " 



Agar Stroke.— 24= hours at 37° d- , ''" , " * * 



Moderate, confluent, raised, *» ^ ^ » * * * *, 



greyish - yellow, translucent ' • - » \ . * ' i 



growth, with ghstening, wavy ' '• 

 or beaten-copper surface and 

 an irregularly lobate edge. „ 



Gelatin Stah.—l days at 22° C. Good ' , 

 filiform growth, confluent at ^ 



top, discrete below, extending 

 to bottom of tube. Raised 

 surface growth, 5 mm. in 

 diameter, with finely lobate ^^ ' ' ^ 



edge. No liquefaction. 

 Broth.^24 hours at 37° C. Moderate Fm. lQ2.-P<^teureUa pseudotuberculosis. 



growth without turbidity and From an agar culture, 24 hours, 37° C. (X 1000). 

 a viscous deposit disinteg- 

 rating on shaking. Later the broth clears and a heavy, flocculo-membranous 

 deposit forms, partly disintegrating on shaking. Incomplete surface and ring 

 growth. 

 Loeffler's Serum. — 24 hours at 37° C. Confluent growth, not so good as on agar. 

 Horse Blood Agar Plate.— 2 days at 37° G. Good growth, but colonies are more compact 

 and less differentiated than on agar. No haemolysis ; whole plate is slightly cleared 

 and browned. 

 Potato.— 1 days at 22° C. A thin yellowish membrane, wliich subsequently terns 



brown. 

 MacConkey Plate.— 24 hours at 37° C. Very slight effuse confluent growth. Colonies 



disappear after a few days, owing presumably to autolysis. 

 Resistance. — Fairly susceptible to inimical agencies. Killed by moist heat at 60'' C, in 



10 minutes. 

 Metabolism. — Aerobe, facultative anaerobe. Opt. temp. 30° C, limits 5-43° C. Growth 

 uninfluenced by serum and glucose, shghtly inhibited by glycerol. No haemo- 

 lysis. 

 Biocheynical. — Acid, no gas, in glucose, maltose, mannitol, salicin, arabinose, xylose, 

 rhamnose, and glycerol within 14 days, sometimes in sucrose. L.M. usually slight 

 alkaU formation ; Indole - ; M.R. + ; V.P. — ; Nitrates + ; NH3 + ; M.B. 

 reduction -|--t- ; HjS -L : Catalase 4-+- 

 Antigenic *S'<n<c<M?-e.— Apparently contains (1) a heat-labile flagellar antigen, which is 

 formed only m cultures grown below about 25° C. ; (2) a heat-stable somatic 

 antigen similar to that of the plague bacillus ; (3) a heat-stable somatic antigen 

 similar to that in Salm.. paratyphi B ; (4) possibly other relatively strain-specific 

 somatic antigens. A plague serum does not protect animals against infection 

 with Past, pseudotuberculosis. 



