810 HEMOPHILUS 



• .1- Resistance. — Similar to H. pertussis. 



' ^ ••" ■; ' Biochemical Activities. — Hsemolysin produced, 



* • * . * V active on red corpuscles of rabbit, dog 



, ' . %\ ^ and guinea-pig. Grows freely on Mac- 



--. * •• ' • •' ., ,<•""" Conkey. No carljohydrates fermented. 



,'*,.>■' ^ ^ -^ ' , Produces marked alkalinity in litmus 



,^ ■ " .. - ' • '• .V _ . .;- milk. Nitrates often reduced. H,S — , 



.„•.•■ *^ • . ' . , ♦ . . . NH3 very slight production or none at 



t. .' ■ . •,. •^.. ■ ■■ aU. Catalase -f -f + . Grows in Koser's 



'■,;': :.'.■>■■ ^. . jV^ / citrate. 



^■^ .w^*- ' _''*'* ^ . Antigenic Structure.- — The surface antigen of 



. ^." • . -•; • .'^ the S form, and its endotoxin, are sero- 



••' «^'* "*-• ' logicallyhomogeneous,and related to the 



'. \ \ 'V- ■• corresponding substances in //. ^jerhtssis 



• ..; . <• , and H. 2}(i>'np€rtussis. 



Fig. lW.-Ha>nophiln.s bro>,cM.septicu.s. Pathogenicity .— Yveqxxent cause of broncho- 



From an agar culture, 24 hours, 37° C. pneumonia in rodents, and of broncho- 



(X 1000). pneumonia compUcattng distemper in 



dogs. Experimentally, intraperitoneal 

 inoculation of guinea-pigs with 0-5 ml. of a 24-hours' broth culture causes death 

 in 24 to 48 hours. Post mortem, there are smaU haemorrhages on the peritoneum, 

 and a viscid translucent exudate forming pseudo-membranes on the hver, spleen, 

 and the less mobile parts of the intestine. The bacilli are easily recovered from 

 the peritoneal cavity, but with difficulty from the blood, hver and lungs. Sub- 

 cutaneous inoculation produces only a local lesion. Feeding and inhalation are 

 without effect. The organism is non -pathogenic to mice. It rapidly loses its 

 virulence in culture. 



REFERENCES 



Alexander, H. E. and Heidelberger, M. (1940) J. exp. Med., 71, 1. 



Anderson, G. and North, E. A. (1943) Aust. J. exp. Biol. med. Sci., 21, 1. 



Anderson, K. and Snow, J. S. (1940) Amer. .J. Path., 16, 269. 



Anderson, L. R. (1931) Amer. J. Hyg., 13, 164. 



Anderson, R. A. and Schultz, 0. T. (1921) J. exp. Med., 33, 653. 



Assis, A. DE. (1926) C. B. Soc. Biol, 95, 1008. 



Baudisch, 0. (1932) Biochem. Z., 245, 265. 



BiELiNG, R. and Weichbrodt, R. (1920) Dtsch. med. Wschr., 46, 1183. 



BoNDi, A. and Flosdorf, E. W. (1943) J. Immunol., 47, 315. 



BoRDET, J. (1912) Zbl. Bakt., 66, 276. 



Bordet, J. and Gengou, 0. (1906) Ann. Inst. Paste ur, 20, 731; (1907) Ibid., 21, 720; 



(1909) Ibid., 23, 415. 

 Bordet, J. and Sleeswyk. (1910) Ann. Inst. Pasteur, 24, 476. 

 Bourn, J. M. (1927) J. infect. Dis., 41, 294. 

 Bradford, W. L. (1938) Amer. J. Path., 14, 377. 



Bradford, W. L. and Slavin, B. (1937) Amer. J. jmbl. HUh, 27, 1277. 

 Brueckner, I. E. and Evans, D. G. (1939) J. Path. Bact., 49, 563. 

 Burnet, F. M. and Timmins, C. (1937) Brit. J. exp. Path., 18, 83. 

 Chandler, C. A., Fothergill, L. D., and Dingle, J. H. (1937) J. exp. Med., 66, 789; 



(1939) J. Bact., 37, 415. 

 Cohen, C. (1909) Ann. Inst. Pasteur, 23, 273. 



Cooper, G. M., Mishulow, L., and Blanc, N. E. (1921) J. Immunol., 6, 25. 

 Cruickshank, J. C. and Freeman, -G. G. (1937) Lancet, ii. 567. 

 Culotta, C. S., Harvey, D. F., and Gordon, E. F. (1935) J. Pediat., 6, 743. 

 Cdnha, R. (1939) Zbl. Bakt., 144, 508 ; (1943) Hospital, 23, 393. 

 Davls, J. D. (1917) J. infect. Dis., 21, 392 ; (1921) Ibid., 29, 178, 187. 

 Delius, W. and Kolle, W. (1897) Z. Hyg. InfektKr., 24, 327. 

 DiBLE, J. H. (1924) J. Path. Bact., 27, 151. 



