356 

 DIMSION OF AXniAL IXDUSTRY. 



glaxi)1-:rs ix waipio \-Arj,i:v. 



Honolulu, Dec. 11. l')12. 



]\Ir. W. ]\I. Giffard. President and Executive Officer, Board of 

 Agriculture and Forestry, Honolulu, T. H. 



Sir : — In compliance with instructions received I returned to 

 Waipio \'alley on November 13th. 



As outlined in my last report it was to be feared that many, 

 if not all, of the horse stock belonging to the Chinese rice planter 

 Akaka was suffering from or at least had become affected with 

 glanders from the seven clinical cases which 1 had destroyed on 

 my previous visit. 



The quarantine of the \'alley has been continued, no horse 

 stock from there being allowed to pass the Hamakua gate leading 

 to W'aimea and only the pack animals belonging to the taro plant- 

 ers, all of which had been found to be sound, had been allowed 

 to carry paiai as far as Paauhau and to return immediately. 



The first step upon my return was therefore to reinspcct these 

 animals, 41 in all, which was done at the mouth of the \'alley 

 at the top of the trail. When the animals fully loaded have 

 climbed this steep trail, a continuous effort requiring about three- 

 fourths of an hour, they are sure to show symptoms, especially a 

 discharge from the nose, which otherwise might remain con- 

 cealed ; but, as stated, they were all found to be sound. 



As far as Akaka was concerned there remained quarantine<l 

 at his place twenty-eight horses and one mule colt. One animal 

 had died since my previous visit and three showed i)hysical 

 .symptoms of the disease not visible when the)' were first ex- 

 amined. It was decided to submit all of these animals ti) the 

 intradermal mallein test, a method which originated with this 

 division. I first injected the saddle horses which my assistant 

 and myself were riding, animals known to be absolutely sound, 

 after which with the same syringe and with mallein out of the 

 same bottle the twenty-eight animals belonging to Akaka were 

 injected. This was no easy matter, as the ojieration is very deli- 

 cate, rec|uiring the use of an extremely fine hypodermic needle. 

 and several of the animals had to be thrown down in order to 

 secure theni sufficiently to allow of the needle being inserted into 

 the hide without ])enetrating it. < )n this point the success of the 

 operation depends. If the needle penetrates the hide no reaction 

 is obtained no matter how severely the animal might be affected. 

 Only two drops is injected, generally on the side or tlu' ntrk. tlir 

 place of operation having first been disinfected. 



The test proved an absolute success. Within one hour from 

 tin- time of injection the mimite swelling, originally the ^ize of a 



