313 



Laws and ordinances may be enacted and enforced and a man 

 thus compelled to have his herd tested, but if he has little knowl- 

 edge of the disease, he will not carry out a thorough cleaning and 

 disinfecting of his premises, without which the tuberculin test is 

 of little avail. The average dairyman sees in the frecjuent clean- 

 ing and disinfecting of his premises only a waste of labor with 

 no apparent results, but let it be said here that his success in main- 

 taining a herd free from tuberculosis is in direct ratio to the fre- 

 quency and thoroughness of his disinfection. 



Bactcriuloi:;ical Examination of the Milk Supply. 



In order to obtain some data on the condition of the milk as it 

 reaches the consumer, a series of bacteriological examinations 

 were made of samples taken from dairies scattered th.roughout the 

 city. The results have been high counts, which indicates that the 

 milk is not being handled with anywhere near the care that is 

 necessary to produce a superior article. 



In all fifteen samples were examined, the results of which are 

 as follows : 



Procedure. 



In each instance a dilution of 1 to 500 was used and with the 

 exception of Samples 1 and 2, in which I made four plates, three 

 plates were made from each sample. The period of incubation 

 varied. 



Results. 



Sample 1 taken from the Honolulu Dairymen's Association con- 

 sisted of 14 hr. old milk from Geo. Holt's dairy. 



Sample 2 consisted of the above milk after it had passed through 

 the purifying apparatus. In subjecting it to this process it was 

 strained through two layers of cotton and cheese cloth, exposed 

 to a steam temperature of 150° F. and an electric temperature of 

 164° F. and then cooled at once to a temperature of 40° F. 



After incubating for 48 hrs. at room temperature (27° C.) 

 examination gave the following : 



Sample 1 10,540,000 bact. per cc. 



2 500 " " " 



This shows admirably the effectiveness of the Groucher sys- 

 tem of purifying milk. 



Sample 3— Geo. Wond 30,000 bact. per cc. 



4— T. F. Farm 2,033,000 " " " 



5— K. Yamashita 1,285,000 " " " 



6— T. F. Farm 2,773,000 " " " 



7— F. Medeiros 1,520,000 " " " 



8— P. Miyakawa 850,000 " " " 



0_R. Davison 750,500 " " " 



10_W. P. Alexander 324,450 " " " 



