54 



This order means that the Division of Plant Inspection of the 

 Board of Agriculture and Forestry will be enabled to keep a close 

 check on all mail importations of plants and 'plant products which 

 hereafter will be inspected only at the Honolulu and Hilo Post 

 Offices. In this manner all such plants will be thoroughly gone 

 over in the effort to keep new plant pests from gaining an en- 

 trance into this Territory. 



Packages containing plants or plant products addressed to 

 places in Hawaii will be accepted for mailing only when the con- 

 tents are plainly marked on the outside. After the contents are 

 inspected at the Hilo or Honolulu Post Offices and are passed 

 as being free from pests they will be forwarded to the ad- 

 dressee. C. S. J. 



AN EXPERIMENT TO DETERMINE THE PRESENCE 

 OF HOG CHOLERA IN THE TERRITORY. 



By Leonard N. Case. 

 Territorml Veterinarian. 



Hog cholera, an infectious and contagious disease of swine is 

 caused by an organism so small as to be ultra-microscopic and 

 which will easily pass through the pores of a filter fine enough to 

 prohibit the passage of all other forms of organic life. This phe- 

 nomenon led to this disease being known as the "filterable virus 

 disease of swine" and also enabled investigators definitely to 

 distinguish this disease from other diseases of swine such as swine 

 plag'ue and the so called mixed infections, the post-mortem lesions 

 of which so closely resemble those usually associated with hog 

 cholera as to be exceedingly confusing. 



Having the means at our disposal of definitely^determining the 

 presence or absence of a disease, an experiment was started with 

 a view to ascertaining whether or not the "filterable virus disease" 

 was present among the swine of this Territory and instructions 

 were given to forward samples of blood from hogs suspected of 

 being sick or dead from hog cholera. 



Consequently on Febraury 14, 1921, a sample of defibrinated 

 blood was received which had been taken from a hog said to be 

 sick from cholera. Several animals had died in this herd and the 

 diagnosis made on post-mortem examinations was reported as 

 ''cholera without a doubt." 



This sample of blood was taken to the bacteriological labora- 

 tories of the Tripler General Hospital at Fort Shafter and care- 

 fully filtered through a Berkefeld filter. Acknowledgement is 

 hereby made of the courtesy and assistance accorded me by the 

 officials in charge of these laboratories. 



The three animals used in the experiment were well bred 

 Berkshires of about eight months of age, the property of the care- 

 taker of the quarantine station and had been in his possession 



