VOL. 4 (1950) 



ENZYMATIC CONDENSATIONS WITH NH.,OH 



307 



was used as a suspension. In Table VII, the hydroxamic acid formation with dodecanoate 

 is described using various fractions. The results are analogous to those obtained with 

 the liver enzyme. 



TABLE VII 



HYDROXAMIC ACID FORMED WITH PANCREATINE, PaRKE-DaVIS 



0.5 g of pancreatine was suspended in 10 ml water, an aliquot was used in experiment i. 20 drops of 

 0.02 molar acetic acid were added and the suspension shaken up. The suspension was centrifuged 

 for half an hour in the cold room. The supernatant was neutralized and used for experiment 2. The 

 residue was resuspended in 0.02 M ammonia buffer with final pH of 8, and used for experiments 

 3 and 4. 



Each tube contained 0.14 ml of 2 M hydroxylamine buffer of pH 6.6, 0.25 ml enzyme solution and 

 o.i ml of o.i M dodecanoate or o.i ml water. The dodecanoate solution had to be warmed up before 

 addition. Incubation for 60 minutes at 37°; hydroxamic acid determination in alcoholic solution. 



30 



20 



HOG LIVER 

 EXTRACT 



PANCREATINE 



6 7 8 S 10 

 CHAIN LENGTH 



Fig. 4. Chain length optimum for liver and pancreas lipase. The conditions for liver extract were as 

 described in Fig. 3, 60 minutes incubation time. Pancreatine, 5% suspension, 0.75 ml in 0.05 M 

 secondary sodium phosphate, 0.45 ml 2 M hydroxylamine, pjj 7, 0.3 ml of 0.05 M fatty acid salts, 



60 minutes incubation. 



References p. jog. 



