342 O. WARBURG et uL, S. B. HENDRICKS VOL. 4 (1950) 



Experiment III, with the same cells, was performed between experiments I and II, the white 

 light being, however, of somewhat lower intensity. Here only one vessel (No. 5) was used; but if we 

 take as y the average value of experiments I and II, that is — 0.85, XQg can be calculated according 

 to equation (3), protocol (i). The readings in vessel (5) were: 



No. 5 



5' white + 5.0 mm 5' white + red -|- 11.5 mm 



5' .. 4- 6.5 ,, 5' „ + 9-5 ., 



5' .. + 6.5 ,, 5' „ + 9.5 „ 



5' .. + 5-5 .. 5' ,. + 13-0 „ 



5' ., + 7-0 .. 5' .. +15-0 .. 



25' white + 30.5 mm 25' white + red + 58.5 mm 



25': H' = 58.5 — 30.5 = + 28 mm 

 and with y = — 0.85 



25' X02 = + 34 /*! 

 The quantum efficiency with the actinometer value of experiments I and II (5.4 /xl O^ per 

 minute) was 



— = lllld = 4.0 for O, 

 •P 34 — 



The total duration of these experiments was 7 hours from the time of initial equilibration until 

 the last yield determination that gave a value — = 4.5 for oxygen, which was obtained at approxi- 

 mately 4 times the compensation point. The final pH in the cell suspensions was 5.4. 



PROTOCOL No. 3 



Comparison of the yield in carbonate-bicarbonate mixtures and in culture medium 



Experiment of VI-i-49. 20° C. 630-660 m/t. Three vessels, in each 7 ml cell suspension, containing 

 200 /il of cells. Cultures centrifuged, then washed once in, and taken up in, carbonate-bicarbonate 

 mixture. Intensity 5.4 /xl Og per minute. 



I. Vessel No. 7. 

 V == 13.824 ml 

 Vf = 7.00 ml 

 ko2 = 0.657 



Gas space air. Solution 85 parts M/io NaHC03+ 15 parts M/io KgCOg; Ph9-2. At compensation 

 point with white light. 



15' white light o 



15' ,, ,, + red light + 11.5 mm 



15' .. .. o 



15' .. .. + .. .. +"-5 ,. 



15' .. — 0-5 : 



Light action 30' + 23 + 0.5 = + 23.5 mm = 15.4 ^l 



I 30'5.4 162 __ 



<P 15-4 15-4 — '- 



II. Vessels Nos. 3 and 5, containing 7 ml culture medium, pn 4-9. with 200 ^1 of cells each. 

 Cultures centrifuged, then washed once in, and taken up in, fresh culture medium. Gas space 5% CO^ 

 in air. Mirror shifted every 10' from one vessel to the other; actinometer 5.4 fd Oj per minute for 

 red light. 



No. 3 



V = 17993 ml 

 Vf = 7000 ,, 



k02 = i-°46 



kco2 = 1-634 



15' white light + ii.o mm 15' white light + red light + 29.5 mm 



15' ,, ,, + red light -f 17.0 ,, 15' ,, ,, + i5-5 .. 



15' .. ,. +10.5 ., 15' .. .. + .. -. +29-0 „ 



15' .. .. + .. .. +16.0 „ 15' „ „ +17-5 .. 



30' H = + II. 5 mm 30' H' = + 25.5 mm 



References p. 346. 



