86 INTERNAL SECRETIONS OF THE OVARY 



tomized rat or mouse. These authors considered that hquor 

 foUicuh was the essential source of the hormone and that the 

 small yields obtained by them from residual ovarian tissue were 

 due to incomplete removal of the follicular fluid. They found 

 that no oestrus-producing substance could be obtained from the 

 solid corpora lutea of lower mammals, but they were later able 

 to demonstrate its presence in the human corpus luteum during 

 the early stages of its development. 



The work of Allen and Doisy and their collaborators has 

 stimulated a great deal of interest in the oestrus-producing 

 hormone, and many authors have since amplified and confirmed 

 their chief results. Frank and his collaborators have prepared 

 the hormone from ovaries, placentae, and corpora lutea, and 

 consider that only one ovarian hormone is present. They have 

 also described a modified technique for the treatment of small 

 quantities of blood (227) by ether extraction after dehydration 

 in anhydrous Na2S04. Courrier (133-9), working on the guinea- 

 pig and rabbit, has described the effects of injection of crude 

 liquor folliculi and of extracts. Other workers in the same field 

 include Papanicolaou (485), Loeb and his collaborators (404-5), 

 Brouha and Simmonett (89-93), Dodds and his collaborators 

 (158), Zondek and Ascheim (646-655), Bugbee and Simond 

 (110-112), Laqueur and his collaborators (343-356), Lipschiitz 

 (373), Loewe (407-419), and Parkes and Bellerby (503-7). These 

 workers have been concerned primarily with studying the 

 distribution of the hormone and with elaborating methods of 

 preparation. 



The preparation in a water-soluble form. The work im- 

 mediately following that of Allen and Doisy was largely based 

 on similar methods of extraction, namely, upon the assumption 

 that the hormone was either fat-soluble or at least close!}/ 

 associated with fats. This assumption implies the use of 

 organic fat solvents in the preparation of extracts. The use of 

 these solvents, however, inevitably brings into solution a large 

 amount of inert material, the presence of which makes such 

 extracts very unsuitable for injection into sensitive animals, 

 particularly the human subject. Various workers, therefore, 

 have endeavoured to obtain active extracts by other methods, or 

 to separate the active material from the oily product. In 



