i68 INTERNAL SECRETIONS OF THE OVARY 



Aschheim and Zondek (38) have also detected the anterior 

 pituitary hormone in the decidua, in the corpus luteum of 

 pregnancy, in navel blood, and in tubal mucous membrane. 



Table ii. — (Estrin and Anterior Pituitary Hormone 

 IN Urine of Pregnancy. 



(After Aschheim and Zondek.) 



Negative results were given by a large number of other tissues 

 and fluids, including male urine. 



(h) ACTION ON THE NORMAL ANIMAL 



Prenatal period. The anterior pituitary hormone would 

 appear to be unable to traverse the placenta — otherwise, in 

 view of its abundance in the blood during pregnancy, the 

 foetuses would presumably be born in a state of sexual maturity. 



Prepubertal period. Smith and Engle (581), using mice rather 

 younger than those used by Zondek and Aschheim, have 

 secured the ovulation reaction at fifteen days of age, five 

 days after the beginning of injections. As a result of the 

 treatment, follicles mature rapidly in the ovary and the antrum 

 appears, together with the cumulus oophorus. Ovulation then 

 takes place and corpora lutea are formed. Smith and Engle 

 imply that the follicles and corpora lutea are all normal, but 

 according to Zondek and Aschheim atretic and blood follicles 

 are also formed. These ovarian changes result in the elaboration 

 of oestrin and in the production of oestrous changes in the 

 accessory organs. Owing, presumably, to the formation of 

 corpora lutea, continued administration does not lead to 

 persistent oestrus, but great hypertrophy of the accessory organs 

 is produced. The continued injection of both anterior pituitary 



