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DISCUSSION 



Wigglesworth: Have you considered the further complication that 

 building up of these reserves may not be a direct effect of the various 

 nutrients, but an effect through the endocrine system? For example, 

 it has long been known that in many adult insects deprived of the 

 corpus allatum the eggs do not develop and do not build up yolk. 

 There is the same effect in protein starvation, where the same 

 histological eff'ects appear. A. S. Johansson (1958. Nature (Loud.), 

 181, 198), working on the milkweed bug, showed that if an active 

 corpus allatimi is implanted in the starved insect there is normal egg 

 development. In other words, the immediate effect of protein feeding 

 seems to be to activate the corpus allatum. The prime deficiency in 

 a protein-starved insect is inactivity of the gland. In your experi- 

 ments it would not necessarily be the corpus allatum; it might be the 

 neurosecretory cells or some other component in the endocrine 

 system. 



Hinton: In bees doesn't the queen substance inhibit the develop- 

 ment of the corpora allata and keep their volume small? 



Maurizio: The degree of development of the ovaries of the honey- 

 bee worker is directly connected with the endocrine system and the 

 queen substance (see references to Butler, Pain and Voogd). It is 



