86 EXPERIMENT STATION RECORD. [Vol. 40 



again centrifuged to recover the remaining organisms. To the precipitate of 

 corpuscles and trypanosomes is added sufficient distilled water to produce com- 

 plete hemolysis of the rat erythrocytes. After about 20 minutes the mixture is 

 centrifuged for about half an hour. The supernatant liquid is then discarded, 

 physiological salt solution is added to the mass of trypanosomes, and the ma- 

 terial vigorously shaken to disintegrate and distribute the trypanosomes evenly 

 through the solution. After centrifuging again, the salt solution is poured off, 

 and an amount of preserving fluid (physiological salt solution and glycerin) 

 equal to about twice the amount of trypanosomes is added. The mixture is then 

 agitated until a uniform suspension is acquired, when it is stored at a low tem- 

 perature until used. 



Experimental evidence is given indicating that the use of distilled water in 

 taking the red blood cells has no detrimental effect on the antigenic value of the 

 trypanosomes. The following advantages of the new method are pointed out: 



" The antigen is freed of all erythrocytes, all the trypanosomes present in the 

 blood are recovered, the keeping quality is Improved, the time consumed is about 

 1J hours, with practically no effort, as compared with 4 or 5 hours, and the 

 antigenic power is increased and the anticomplementary action diminished." 



Bovine tuberculosis, A. C. Fontes (Tuberculosa Bovina. Rio de Janeiro: 

 Author, 19 H. pp. 82). — This is a report presented to the executive committee of 

 the Live Stock Congress in Rio de Janeiro, May, 1917. A review of literature 

 on the subject of bovine tuberculosis Is given, together with statistical data col- 

 lected at slaughterhouses in regard to the relative localization of the lesions 



Prophylaxis of bovine tuberculosis in Argentina. A. F. Beyro (An. Soc. 

 Rural Argentina, 52 (1918), Nos. /. /</;. 12-21; ..'. pp. S0-S7 ; 3, pp. 175-188; Rcr. 

 Soc. Med. Vet. [Buenos Aires], 3 (MIS), No. 5, pp. 189-181; abs. in Yet. R< v., .: 

 (1918), Xo. 4- P- 487). — The author discusses tin' extent of bovine tuberculosis in 

 Argentina, the economic loss caused by the disease, and plans to serve as a ba>is 

 for an efficient prophylaxis of the disease. From available figures for the year 

 1015 and later, it is estimated that about ."..•_' per cent of the cattle in Argentina 

 are tubercular and that the percentage is Increasing. The prophylactic meas- 

 ures suggested are similar to those in use in the United States. 



Tuberculosis in the camel, F. E. Mason (Jour. Compar. Path, and Tlicr., 31 

 (1018), No. 2, pp. 100-102; abs. in 1"< /. /.'< »., J (1918), No. !,, p. 4S9).— Continu- 

 ing the work previously noted (E. S. R., 37, p. 690), the author lias reported a 

 case of congenital tuberculosis in the camel. Tubercular lesions were found in 

 an aborted fetus from which typical tubercle bacilli were isolated. The tuber- 

 culin test gave a positive reaction with the cow camel which had aborted. A 

 case of tuberculosis in an Algerian camel is also noted. 



[Live stock diseases in Louisiana] (Bien. Rpt. Live Stork Sanit. Bd. La., 5 

 (1917-18), pp. 16-60, figs. 13). — Descriptions are given of several of the more 

 important infectious live-stock diseases in Louisiana, including anthrax, black- 

 leg, staggers, glanders, hog cholera, hemorrhagic septicemia, and rabies. In- 

 formation in regard to anthrax is given in the form of a popular questionnaire 

 by Dalrymple and Flower. 



Common diseases of the digestive organs of horses and cattle. J. H. Reed 

 (Ontario Dept. Agr. Bui. 264 (1918), pp. 39). — A practical discussion for the 

 stock owner. 



Contagious abortion of cattle (Kansas Sta. Circ. 69 (191S), pp. 16). — This 

 circular summarizes available information on the subject of contagious abortion 

 of cattle. The nature, cause, symptoms, complications, and methods of spread of 

 the disease are outlined. The control of the disease is discussed fully under 

 the three principles of preventing the dissemination of infection, developing herd 

 immunity, and treating affected animals. 



