882 EXPERIMENT STATION RECORD. 



[Vol. 40 



I. Gas production by Bacterium pullorum (pp. 65, 66). — Gaa production by 

 B. pullorum was shown to depend upon whether the cultures are grown In 

 glucose extract or In glucose Infusion broth, more being formed in the latter 

 medium. Propagating cultures for many years on artificial media has not been 

 found to cause a loss in their gas-producing ability. 



II. B. pullorum infections in adult stock (pp. 66, 671. — Anaerogenic strains of 

 B. pullorum have been isolated from adult fowls experiencing acute or subacute 

 infections simulating fowl typhoid in both clinical symptoms and pathological 

 alterations in the tissues (K. S. R., 38, p. 889). It is proposed to classify two 

 subspecies of B. pullorum as follows: (1) B. pullorum a, aerogenic and patho- 

 genic for chicks, and (2) B. pullorum /3, anaerogenic and pathogenic for adult 

 stock only. 



III. Correlation between sucrose fermentation anA immunizing pmcer of 

 Bacillus avisepticut (pp. 68, 69). — Strain 52 of B. arisepticus, described in an 

 earlier bulletin ( E. S. R., 27, p. 583), has been found to be unlike other strains 

 of tbe Organism in that It produces no acid with sucrose. As this is correlated 

 with the ability to produce in rabbits resistance to powerful Infection with 

 virulent cultures, an ability possessed by no other strain, the question is raised 

 as to the possible significance of the fermentative reaction in relation to im- 

 munity production. 



Immunization products and indications for their use, O. MUKBAY (Jour. 

 Amer. Vet. Med. Assoc. 55 {1019), No. 1, pp. 68-76).— This is a general discus- 

 sion of active and passive immunity and the means of acquiring immunity to 

 certain diseases. 



The germicidal power of antiseptic oils and of substances dissolved in oil, 

 P. D. IfcMASTEB (Jour. Infect. Disrates, %h (1919), So. 4. pp. S78S85 ) .—The 

 following method has been developed to determine quantitatively the germi- 

 cidal power of antiseptic oils and substances dissolved in oils; 



Agar slants, well drained of the water • ■ f condensation, are inoculated with 

 Bacillus typhosus over an area of not more than 1 cm. and well above the mid- 

 dle of the tube. After incubating f< »r 24 hours, the tubes are tilled with the oil 

 to be tested and incubated for 24 hours at 37.5* C, after which the oils are poured 

 off and the tubes washed twice with sterile salt solution. A transplant of the 

 organism is then made to a tube containing 8 cc. standard broth, and readings 

 of this tube are made after 24 hours' Incubation. Phenol dissolved in mineral 

 oil was found to have a germicidal value approaching that of its value in 

 water, and thus to be suitable as an arbitrary standard for comparison when 

 testing the activity of ether nils and oil-soluble BUbStan< 



The coefficients of certain disinfectants in oils as determined by this method 

 are reported as follows: Phenol In paraffin oil 1, iodin in paraffin oil 1, phenol 

 In cottonseed oil 0.16, paracresol in paraffin oil 1.55, guaiacol In paraffin oil 

 0.44, dichloraniin-T in 10 per cent chlorinated eucalyptol in chlorinated paraffin 

 oil 8, and dichloramin-T in plain paraffin oil 1. 



The author considers the method as so far developed satisfactory, except 

 that any advantage which a substance might possess in the shape of rapidity of 

 action Is largely masked by the 24-hour period of exposure used. 



Proflavin oleate in the treatment of open wounds, C. RKRKEtr.v and V. 

 BoNNfcY {Brit. Med. Jour.. No. 909% (1919). pp. 152. 7.5.'?).— Attention is .-ailed 

 to the value of proflavin oleate in the treatment of wounds in which an ex- 

 ceedingly tender, raw surface of considerable extent exists. The oleate oint- 

 ment can be applied directly to the wound and covered with a single layer of 

 gauze, no bandage being required to keep it in contact with the wound. 



