774 EXPERIMENT STATION RECORD. 



The serological diagnosis of glanders in asses, hinnies, mules, and horses 

 with the so-called nonspecific inhibition of complement fixa.tion deviation, 

 W. Pfkileb and G. Webee (Ztschr. Infektionskrank. u. Hyg. Eaustiere, 16 

 (1915), No. 5, pp. Sll-323). — The object of this investigation was to determine 

 whether it is possible by a modified complement fixation or another serological 

 method to distinguish between specific and nonspecific antibodies in the sera 

 of animals. See also notes by Schiitz and Waldemann (E. S. R., 33, p. 479). 



It is concluded that this can be accomplished with the conglutination reaction 

 (E. S. R., 38, p. 479). In the investigation 6,500 sera were examined, among 

 which tliere were 5 whicli did not give a specific deviation of complement. The 

 agglutination values of these animals were in no case more than 500, but tliey 

 could be regarded as suspects, whereas with the conglutination method only 1 

 of the 5 horses could be so regarded. This animal, on autopsy, was found 

 glandered. 



With the sera of 14 asses it was not possible to say whether glanders was 

 present from either the complement fixation or agglutination test, but in one 

 case it was noted with the conglutination test. 



Necrobacillosis, A. T. Kinsley (Amer. Jour. Vet. Med., 10 (1915), No. 9, 

 pp. 609, 610, 655, 656). — It is pointed out that necrobacillosis is a name given 

 to a group of pathologic conditions caused by Bacillus nccrophoms and charac- 

 terized by inflammation and necrosis. This includes lip-and-leg ulceration and 

 foot rot in sheep ; stomatitis, enteritis, pneumonitis, hepatitis, splenitis, and 

 dermatitis in swine ; necrotic dermatitis in the horse ; stomatitis in calves ; ano- 

 vulvitis in cattle, etc. An outbreak of ano-vulvitis in a feed lot of 115 head of 

 heifers in Missouri in March, 1915, is reported upon. 



The effect of quinin on rabies, G. Keumwiede, Je., and Alice G. Mann 

 (Jour. Infect. Diseases, 16 (1915), No. 1, pp. 2If, 25).— "Of four dogs treated 

 with quinin, one showed a prolonged period of incubation and another showed a 

 remission in the course of the disease, in either case, however, not beyond vari- 

 ations in the natural course of the disease. The fact that these variations oc- 

 curred in the two of four dogs receiving the largest amounts of quinin is prob- 

 ably only a coincidence. No influence was observed on the period of incubation 

 in rabbits." 



The bovine hemoglobinuria of Chile, a disease due to spirochetif orm para- 

 sites, J. Bliee (Compt. Rend. Acad. Sci. [Paris], 159 (1914), ^^o. 24, pp. 815- 

 817). — A brief report upon a disease of cattle occurring in the region of San- 

 tiago, Chile, and known as " Meada de Sangre," which has often been confused 

 with anthrax. The death of the affected animal nearly always takes place 

 within 48 hours. 



Agglutination studies of milk from cows affected with contagious abor- 

 tion, L. H. Cooledge (Abs. in Science, n. ser., 42 (1915), No. 1080, p. 352). — The 

 author's investigations were carried on with milk from a herd having a record 

 of frequent abortion and in which a high percentage of the animals had re- 

 peatedly given positive complement fixation and agglutination tests. The milk 

 of 18 out of 61 cows, each quarter of which had been examined at intervals 

 during a period of six months, gave a positive agglutination test with Bacillus 

 abortus in one or more quarters at some time or during this period. 



" The power of the milk of one quarter to agglutinate the abortion bacterium 

 has been observed to spread to another quarter and finally to all four. It has 

 also been observed to gradually die out. Milk drawn at about the middle of 

 the milking has the strongest agglutinating reaction." 



In an attempt to demonstrate the presence of B. abortus in milk that aggluti- 

 nates the organism, the milk of 14 of 18 quarters produced lesions in guinea 

 pigs typical of the pure culture of this organism. " In the seven cows whose 



