20 EXPEEIMENT STATION RECORD. 



pp. 328-331, fig. J).— A ixjlemic with Hcrlitzka (E. S. R.. 28, p. 407) in regard 

 to priority." 



A dilution colorimeter and some remarks in reference to the experimental 

 error in comparing- colors, li. V. Stanfokd (Hoppe-Seyler's Ztschr. Physiol. 

 Chcm., 87 (1913), No. 3, pp. 159-170, pi. 1, figs. 2).— The apparatus is illustrated 

 and sectional views of it are shown. 



A color reaction for proteins, L. Lewin (Med. KUnik, 9 (1913), No. 28, p. 

 1122; abs. in ZentU. Expt. Med., 4 {1913), No. IJf, p. 635).— Triformoxim 

 ( trioximiuimomethylen, Kahlbaum, 0.1 to 0.15 gm. dissolved in 100 gm. of com- 

 mercial sulphuric acid) yields with egg albumin, serum albumin, casein, peptones, 

 nucleoproteins and other proteins, and papain, a coagulation ring at the point of 

 contact with the reagent. The ring on agitation becomes a violet coloration. 

 With this sensitive reaction traces of proteins can be detected in saliva. Indol 

 gives the same reaction. 



About the detection of histidin, K. Inoute (Hoppe-Seyler's Ztschr. Physiol. 

 Chem., 83 (1913), No. 1, pp. 79-82). — The method depends on the formation of 

 an azo coloring matter. If diazobenzolsulphonie acid, in the presence of an 

 excess of sodium carbonate, is added to free histidin a cherry-red coloration 

 is obtained. The reaction is sensitive to a dilution of 1:100,000. Tyrosin 

 when present may be rendered inactive by shaking with benzoyl chlorid. 



The detection of saponins. III, J. Eijhle (Ztschr. Untersiich. Nahr. u. 

 Gcnussmtl., 27 (1914), No. 1-3, pp. 192-200).— Bight coloring matters intended 

 for coloring lemonade, effervescent beverages, etc., did not have any hemolytic 

 properties. Two sapogenins prepared from the saponins of soap root and soap 

 bark were found to be four times as soluble in ether as the saponins from which 

 they were obtained. Both sapogenins, as well as that prepared from guaiac 

 bark, showed slight but definite hemolytic properties. The saponins, saponic 

 acid, and acid saponins from guaiac bark were still hemolytic in dilutions of 

 1 : 5,000. 



It is concluded that the addition of saponin to effervescent drinks should not 

 be permitted. See also previous work (E. S. R., 20, p. 612; 27, p. 505). 



Detection and differentiation of poisonous and nonpoisonous saponins by 

 hemolysis and the conclusions to be drawn therefrom, J. Haleerkaxn (Abs. 

 in Apoth. Ztg., 28 (1913), No. 13, p. 118; CentU. Bakt. [etc.], 1. Abt., Ref., 59 

 (1913), No. 1, p. 12). — The poisonous saponins when combined with cholesterol 

 become nontoxic. Saponins act upon the membranes of the red blood cor- 

 puscles and consequently allow an escape of the hemoglobin. Perhaps hemolysis 

 is due to the action of the cholesterol. According to these investigations tox- 

 icity and hemolysis run parallel because a saponin which exerts no or only a 

 slight hemolysis has only a slight action on man and animals. For detecting 

 saponins, a 1 per cent suspension of blood corpuscles is exposed to saponin solu- 

 tions of various strengths. The method can be used for estimating saponins in 

 mineral waters. 



Determination of g-lycerol in wine, with especial reference to a new 

 simple method, S. Rothenfusser (Ztschr. Untersuch. Nahr. u. Genussmtl., 

 26 (1913), No. 10, pp. 535-5U, fig. i). — The method consists essentially of 

 oxidizing the glycerol to oxalic acid and titrating the latter with a standard 

 solution of potassium permanganate. 



A new method for differentiating' human from cow's milk and a contri- 

 bution to the study of the lipolytic milk ferments, H. Davidsohn (Ztschr. 

 Kindcrhcilk., Orig., 8 (1913), No. 1, pp. 14-23; abs. in Ztschr. Immunitdtsf. u. 

 Expt. Ther., II, Rcf., 7 (1913), No. 8, p. 383).— On adding 1 to 2 drops of 



"Ber. Deut. Bot. Gesell., 25 (1907 », No. 8, pp. 416-424, pi. 1. 



